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Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using AIRE Monoclonal antibody (66262-1-Ig)

    WB analysis of human spleen using 66262-1-Ig (same clone as 66262-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66262-1-Ig (AIRE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66262-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using AIRE Monoclonal antibody (66262-1-Ig)

    WB analysis of pig spleen using 66262-1-Ig (same clone as 66262-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66262-1-Ig (AIRE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66262-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using AIRE Monoclonal antibody (66262-1-Ig)

    WB analysis of human spleen using 66262-1-Ig (same clone as 66262-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66262-1-Ig (AIRE Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66262-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AIRE Monoclonal antibody (66262-1-Ig)

    IF Staining of HeLa using 66262-1-Ig (same clone as 66262-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AIRE antibody (66262-1-Ig, Clone: 1A3C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66262-1-PBS in a different storage buffer formulation.

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AIRE Monoclonal antibody, PBS Only

AIRE Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66262-1-PBS
Clone No.1A3C3

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

PGA1, APSI, APS1, APECED, AIRE1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66262-1-PBS targets AIRE in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIRE fusion protein Ag19103 相同性解析による交差性が予測される生物種
Full Name autoimmune regulator
Calculated molecular weight 545 aa, 58 kDa
Observed molecular weight 58-69 kDa
GenBank accession numberBC137270
Gene Symbol AIRE
Gene ID (NCBI) 326
RRIDAB_2881649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43918
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.