AK1 Polyclonal antibody

Name: AK1 Polyclonal antibody
Brand: Proteintech
SKU: 14978-1-AP

AK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14978-1-AP

Synonyms

adenylate kinase 1, Adenylate kinase isoenzyme 1, AK 1, AK1, ATP AMP transphosphorylase 1, Myokinase

Validation Data Gallery

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AllWBIHC

WB analysis using 14978-1-AP
mouse heart lysates were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14978-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 14978-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14978-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14978-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse heart using 14978-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14978-1-AP (AK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 14978-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14978-1-AP (AK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 14978-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected in	mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below

Product Information

14978-1-AP targets AK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AK1 fusion protein Ag6890 相同性解析による交差性が予測される生物種
Full Name	adenylate kinase 1
Calculated molecular weight	22 kDa
Observed molecular weight	22 kDa
GenBank accession number	BC001116
Gene symbol	AK1
Gene ID (NCBI)	203
RRID	AB_2225148
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for AK1 antibody 14978-1-AP	Download protocol
IHC protocol for AK1 antibody 14978-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	J Proteome Res Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts Authors - Bridgette Hartley View Article
mouse	WB	Eur J Pharmacol Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection. Authors - Gerald Wölkart View Article
	WB	PLoS One Adenylate kinase 1 overexpression increases locomotor activity in medaka fish Authors - Michiyo Maruyama View Article
	WB	Cell Res Hierarchical activation of compartmentalized pools of AMPK depends on severity of nutrient or energy stress. Authors - Yue Zong View Article
human,mouse	WB	EMBO J Aberrant DNA N6 -methyladenine incorporation via adenylate kinase 1 is suppressed by ADAL deaminase-dependent 2'-deoxynucleotide pool sanitation Authors - Shaokun Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AK1 Polyclonal antibody

AK1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 14978-1-AP

WB analysis of human heart using 14978-1-AP

WB analysis using 14978-1-AP

WB analysis of mouse skeletal muscle using 14978-1-AP

WB analysis of human heart using 14978-1-AP

IHC staining of mouse heart using 14978-1-AP

IHC staining of mouse heart using 14978-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts

Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection.

Adenylate kinase 1 overexpression increases locomotor activity in medaka fish

Hierarchical activation of compartmentalized pools of AMPK depends on severity of nutrient or energy stress.

Aberrant DNA N6 -methyladenine incorporation via adenylate kinase 1 is suppressed by ADAL deaminase-dependent 2'-deoxynucleotide pool sanitation