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  • Featured Product
  • KD/KO Validated

AK2 Monoclonal antibody, PBS Only

AK2 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H2C10

Cat no : 66127-1-PBS

Synonyms

adenylate kinase 2, ADK2, AK 2, AK2, ATP AMP transphosphorylase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AK2 Monoclonal antibody (66127-1-Ig)

    WB analysis using 66127-1-Ig (same clone as 66127-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using AK2 Monoclonal antibody (66127-1-Ig)

    WB analysis of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)

    WB result of AK2 antibody (66127-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AK2 transfected HeLa cells. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AK2 Monoclonal antibody (66127-1-Ig)

    WB analysis of HEK-293 using 66127-1-Ig (same clone as 66127-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human liver using 66127-1-Ig (same clone as 66127-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human liver using 66127-1-Ig (same clone as 66127-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human heart using 66127-1-Ig (same clone as 66127-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human heart using 66127-1-Ig (same clone as 66127-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AK2 Monoclonal antibody (66127-1-Ig)

    IF Staining of HepG2 using 66127-1-Ig (same clone as 66127-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66127-1-Ig (AK2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AK2 Monoclonal antibody (66127-1-Ig)

    IF Staining of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)

    Immunofluorescent analysis of HeLa cells using 66127-1-Ig (AK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using AK2 Monoclonal antibody (66127-1-Ig)

    FC experiment of HepG2 using 66127-1-Ig (same clone as 66127-1-PBS)

    1X10^6 HepG2 cells were stained with .2ug AK2 antibody (66127-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66127-1-PBS targets AK2 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AK2 fusion protein Ag17911 相同性解析による交差性が予測される生物種
Full Name adenylate kinase 2
Calculated molecular weight 26 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC009405
Gene symbol AK2
Gene ID (NCBI) 204
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.