WB Figures
WB analysis using 66127-1-Ig (same clone as 66127-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)
WB result of AK2 antibody (66127-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AK2 transfected HeLa cells. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66127-1-Ig (same clone as 66127-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of human liver using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of rat liver using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of rat liver using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of rat heart using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of rat heart using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 66127-1-Ig (same clone as 66127-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AK2 antibody (66127-1-Ig, Clone: 3H2C10 ) at dilution of 1:5024 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66127-1-Ig (same clone as 66127-1-PBS)
Immunofluorescent analysis of HepG2 cells using 66127-1-Ig (AK2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)
Immunofluorescent analysis of HeLa cells using 66127-1-Ig (AK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.
CYTOMETRIC BEAD ARRAY Figures
Cytometric bead array standard curve of MP50407-1
Cytometric bead array standard curve of MP50407-1, AK2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66127-1-PBS. Detection antibody: 66127-2-PBS. Standard:Ag17911. Range: 0.195-50 ng/mL.