Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of human placenta using 12562-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 12562-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12562-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12562-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12562-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 12562-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 12562-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12562-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12562-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 12562-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 12562-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 12562-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 12562-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12562-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12562-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12562-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12562-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 12562-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 12562-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12562-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12562-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12562-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 12562-1-AP
IP result of anti-AK3 (IP:12562-1-AP, 3ug; Detection:12562-1-AP 1:500) with mouse kidney tissue lysate 4400ug.
IHC staining of human pancreas cancer using 12562-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12562-1-AP (AK3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 12562-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 12562-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 12562-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12562-1-AP (AK3 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

AK3 Polyclonal antibody

AK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12562-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

AKL3L1, AKL3L, AK6, AK3L1, AK 3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human placenta tissue, BxPC-3 cells, HEK-293 cells, HepG2 cells, human kidney tissue, L02 cells, MCF-7 cells, mouse heart tissue, mouse kidney tissue, Y79 cells
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human pancreas cancer tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

12562-1-AP targets AK3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AK3 fusion protein Ag3146 相同性解析による交差性が予測される生物種
Full Name	adenylate kinase 3
Calculated molecular weight	227 aa, 26 kDa
Observed molecular weight	26 kDa
GenBank accession number	BC013771
Gene Symbol	AK3
Gene ID (NCBI)	50808
RRID	AB_2305373
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UIJ7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AK3(Adenylate kinase 3) is also named as AK3L1, AK6, AKL3L and belongs to the adenylate kinase family. It is located in the mitochondrial matrix and is a GTP:AMP phosphotransferase(PMID:2546792). AK3 can be detected AK3 at an apparent molecular mass of 28 kD by western blot analysis and subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction(PMID:11485571). This antibody may also recognize AK4 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for AK3 antibody 12562-1-AP	Download protocol
IHC protocol for AK3 antibody 12562-1-AP	Download protocol
IF protocol for AK3 antibody 12562-1-AP	Download protocol
IP protocol for AK3 antibody 12562-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Med Rep MicroRNA‑214 upregulates HIF‑1α and VEGF by targeting ING4 in lung cancer cells. Authors - Yue Li View Article
human	WB	J Gene Med MiR-96-5p promotes breast cancer migration by activating MEK/ERK signaling. Authors - Wei-Yan Qin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human placenta using 12562-1-AP

WB analysis of BxPC-3 using 12562-1-AP

WB analysis of mouse kidney using 12562-1-AP

WB analysis of HEK-293 using 12562-1-AP

WB analysis of HEK-293 using 12562-1-AP

WB analysis of human placenta using 12562-1-AP

WB analysis of human placenta using 12562-1-AP

WB analysis of mouse kidney using 12562-1-AP

WB analysis of mouse kidney using 12562-1-AP

WB analysis of BxPC-3 using 12562-1-AP

WB analysis of BxPC-3 using 12562-1-AP

WB analysis of L02 using 12562-1-AP

WB analysis of L02 using 12562-1-AP

WB analysis of MCF-7 using 12562-1-AP

WB analysis of MCF-7 using 12562-1-AP

WB analysis of HepG2 using 12562-1-AP

WB analysis of HepG2 using 12562-1-AP

WB analysis of mouse heart using 12562-1-AP

WB analysis of mouse heart using 12562-1-AP

WB analysis of mouse kidney using 12562-1-AP

WB analysis of human kidney using 12562-1-AP

WB analysis of Y79 using 12562-1-AP

IP experiment of mouse kidney using 12562-1-AP

IHC staining of human pancreas cancer using 12562-1-AP

IHC staining of human heart using 12562-1-AP

IHC staining of human heart using 12562-1-AP

IF Staining of HepG2 using 12562-1-AP

AK3 Polyclonal antibody

AK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MicroRNA‑214 upregulates HIF‑1α and VEGF by targeting ING4 in lung cancer cells.

MiR-96-5p promotes breast cancer migration by activating MEK/ERK signaling.