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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using AKAP11-Specific Polyclonal antibody (30552-1-AP)

    WB analysis of MCF-7 using 30552-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 30552-1-AP (AKAP11-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using AKAP11-Specific Polyclonal antibody (30552-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30552-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30552-1-AP (AKAP11-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

AKAP11-Specific Polyclonal antibody

AKAP11-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30552-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

AKAP11, A-kinase anchor protein 11, AKAP220, AKAP-11, AKAP 220

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30552-1-AP targets AKAP11-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP11-Specific fusion protein Ag31539 相同性解析による交差性が予測される生物種
Full Name A kinase (PRKA) anchor protein 11
Calculated molecular weight 211 kDa
Observed molecular weight250 kDa
GenBank accession numberNM_016248
Gene Symbol AKAP11
Gene ID (NCBI) 11215
RRIDAB_3086360
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKA4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKAP11, also named as AKAP220, KIAA0629 and PRKA11, belongs to the AKAP110 family. AKAP11 binds to type II regulatory subunits of protein kinase A and anchors/targets them. The antibody is specific to AKAP11.

Protocols

Product Specific Protocols
WB protocol for AKAP11-Specific antibody 30552-1-APDownload protocol
IHC protocol for AKAP11-Specific antibody 30552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols