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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKAP12 Polyclonal antibody (25199-1-AP)

    WB analysis using 25199-1-AP (same clone as 25199-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using AKAP12 Polyclonal antibody (25199-1-AP)

    WB analysis of rat brain using 25199-1-AP (same clone as 25199-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AKAP12 Polyclonal antibody (25199-1-AP)

    IHC staining of mouse brain using 25199-1-AP (same clone as 25199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AKAP12 Polyclonal antibody (25199-1-AP)

    IHC staining of mouse brain using 25199-1-AP (same clone as 25199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using AKAP12 Polyclonal antibody (25199-1-AP)

    IHC staining of human colon using 25199-1-AP (same clone as 25199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using AKAP12 Polyclonal antibody (25199-1-AP)

    IHC staining of human placenta using 25199-1-AP (same clone as 25199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25199-1-AP (AKAP12 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using AKAP12 Polyclonal antibody (25199-1-AP)

    IHC staining of human placenta using 25199-1-AP (same clone as 25199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25199-1-AP (AKAP12 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

AKAP12 Polyclonal antibody, PBS Only

AKAP12 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25199-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25199-1-PBS targets AKAP12 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP12 fusion protein Ag17769 相同性解析による交差性が予測される生物種
Full Name A kinase (PRKA) anchor protein 12
Calculated molecular weight 1782 aa, 191 kDa
Observed molecular weight 200-300 kDa
GenBank accession numberBC157067
Gene Symbol AKAP12
Gene ID (NCBI) 9590
RRIDAB_2879954
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02952
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

AKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). AKAP12 expression is known to be suppressed in several human malignancies including breast, prostate, gastric and colon cancers. AKAP12 has three isoforms, namely AKAP12A, AKAP12B and AKAP12C, with molecular weights of 305 kDa, 287 kDa and 250 kDa respectively. (PMID: 30385176, PMID: 15258566)