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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AKAP3 Recombinant antibody

AKAP3 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230278C3

Cat no : 82986-1-RR

Synonyms

AKAP-3, AKAP110, AKAP 3, AKAP 110, A kinase anchor protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKAP3 Recombinant antibody (82986-1-RR)

    WB analysis using 82986-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82986-1-RR (AKAP3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82986-1-RR

    Affinity and Kinetic Characterization of 82986-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82986-1-RR against Human AKAP3 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82986-1-RR targets AKAP3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AKAP3 fusion protein Ag33655 相同性解析による交差性が予測される生物種
Full Name A kinase (PRKA) anchor protein 3
Calculated molecular weight 95 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC047535
Gene symbol AKAP3
Gene ID (NCBI) 10566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

Protocols

Product Specific Protocols
WB protocol for AKAP3 antibody 82986-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols