• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AKAP8 Polyclonal antibody

AKAP8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31542-1-AP

Synonyms

A kinase anchor protein 8, A kinase anchor protein 95 kDa, AKAP 8, AKAP 95, AKAP8, AKAP95, DKFZp586B1222

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using AKAP8 Polyclonal antibody (31542-1-AP)

    WB analysis using 31542-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31542-1-AP (AKAP8 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive WB detected inA549 cells, HL-60 cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31542-1-AP targets AKAP8 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP8 fusion protein Ag35816 相同性解析による交差性が予測される生物種
Full Name A kinase (PRKA) anchor protein 8
Calculated molecular weight76kDa，692aa
Observed molecular weight 95 kDa
GenBank accession numberNM_005858.3
Gene symbol AKAP8
Gene ID (NCBI) 10270
RRIDAB_3670031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKAP8 (A-kinase anchor protein 8), also called AKAP95, is a A-kinase anchor protein that directs activity of protein kinase A by tethering the enzyme near its physiologic substrates (PMID: 9473338; 10601332). It is associated with chromatin and nuclear matrix which has a remarkable activity in promoting chromatin transcription (PMID: 31980632), and it is also required for cell cycle G2/M transition and histone deacetylation during mitosis (PMID: 16980585).

Protocols

Product Specific Protocols
WB protocol for AKAP8 antibody 31542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols