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- KD/KO Validated
AKAP8L Polyclonal antibody
AKAP8L Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 10103-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
Product Information
10103-1-AP targets AKAP8L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AKAP8L fusion protein Ag0145 相同性解析による交差性が予測される生物種 |
| Full Name | A kinase (PRKA) anchor protein 8-like |
| Calculated molecular weight | 72 kDa |
| Observed molecular weight | 72 kDa |
| GenBank accession number | BC000713 |
| Gene symbol | AKAP8L |
| Gene ID (NCBI) | 26993 |
| RRID | AB_2258197 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AKAP8L, also named as NAKAP, or NAKAP95, is a 646 amino acid protein, which contains two C2H2 AKAP95-type zinc fingers and belongs to the AKAP95 family. AKAP8L localizes in the nucleus matrix and is expressed in the brain cortex. AKAP8L could play a role in constitutive transport element (CTE)-mediated gene expression. AKAP8L may be involved in nuclear envelope breakdown and chromatin condensation. It also may regulate the initiation phase of DNA replication when associated with TMPO-beta.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AKAP8L antibody 10103-1-AP | Download protocol |
| IF protocol for AKAP8L antibody 10103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Retrovirology The role of A-kinase anchoring protein 95-like protein in annealing of tRNALys3 to HIV-1 RNA.
| ||
RNA Biol hnRNPH1-MTR4 complex-mediated regulation of NEAT1v2 stability is critical for IL8 expression.
|
