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  • Featured Product
  • KD/KO Validated

AKR1A1 Polyclonal antibody

AKR1A1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15054-1-AP

Synonyms

AKR1A1, Alcohol dehydrogenase [NADP+], Aldehyde reductase, ALDR1, ALR, ARM, DD3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using AKR1A1 Polyclonal antibody (15054-1-AP)

    WB analysis of HeLa using 15054-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using AKR1A1 Polyclonal antibody (15054-1-AP)

    WB analysis of mouse lung using 15054-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using AKR1A1 Polyclonal antibody (15054-1-AP)

    WB analysis of L02 using 15054-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using AKR1A1 Polyclonal antibody (15054-1-AP)

    WB analysis of human liver using 15054-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using AKR1A1 Polyclonal antibody (15054-1-AP)

    IP experiment of HeLa using 15054-1-AP

    IP result of anti-AKR1A1 (IP:15054-1-AP, 4ug; Detection:15054-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AKR1A1 Polyclonal antibody (15054-1-AP)

    IF Staining of HeLa using 15054-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15054-1-AP (AKR1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, human liver tissue, L02 cells, mouse lung tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15054-1-AP targets AKR1A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKR1A1 fusion protein Ag7037 相同性解析による交差性が予測される生物種
Full Name aldo-keto reductase family 1, member A1 (aldehyde reductase)
Calculated molecular weight 37 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000670
Gene symbol AKR1A1
Gene ID (NCBI) 10327
RRIDAB_2224138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AKR1A1 antibody 15054-1-APDownload protocol
IF protocol for AKR1A1 antibody 15054-1-APDownload protocol
IP protocol for AKR1A1 antibody 15054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC,IP

Nature

Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury.

Authors - Hua-Lin Zhou
  • KO Validated
View Article
mouseWB

J Med Chem

Identification of a Selective SCoR2 Inhibitor That Protects Against Acute Kidney Injury

Authors - Hua-Lin Zhou
View Article
mouseWB

J Biol Chem

AKR1A1 is a novel mammalian S-nitroso-glutathione reductase.

Authors - Colin T Stomberski
  • KO Validated
View Article