Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, L02 cells, mouse lung tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
Product Information
15054-1-AP targets AKR1A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AKR1A1 fusion protein Ag7037 相同性解析による交差性が予測される生物種 |
| Full Name | aldo-keto reductase family 1, member A1 (aldehyde reductase) |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC000670 |
| Gene Symbol | AKR1A1 |
| Gene ID (NCBI) | 10327 |
| RRID | AB_2224138 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14550 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AKR1A1 antibody 15054-1-AP | Download protocol |
| IHC protocol for AKR1A1 antibody 15054-1-AP | Download protocol |
| IF protocol for AKR1A1 antibody 15054-1-AP | Download protocol |
| IP protocol for AKR1A1 antibody 15054-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury.
| ||
J Med Chem Identification of a Selective SCoR2 Inhibitor That Protects Against Acute Kidney Injury | ||