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AKR1B1 Monoclonal antibody

AKR1B1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F11G5

Cat no : 67498-1-Ig

Synonyms

ADR, AKR1B1, Aldehyde reductase, Aldose reductase, ALDR1, ALR2, AR

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using AKR1B1 Monoclonal antibody (67498-1-Ig)

    WB analysis of HEK-293 using 67498-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AKR1B1 Monoclonal antibody (67498-1-Ig)

    WB analysis of HeLa using 67498-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AKR1B1 Monoclonal antibody (67498-1-Ig)

    WB analysis of HepG2 using 67498-1-Ig

    WB result of AKR1B1 antibody (67498-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR1B1 transfected HepG2 cells.

  • Western Blot (WB) analysis of HepG2 cells using AKR1B1 Monoclonal antibody (67498-1-Ig)

    WB analysis of HepG2 using 67498-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using AKR1B1 Monoclonal antibody (67498-1-Ig)

    WB analysis of Jurkat using 67498-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using AKR1B1 Monoclonal antibody (67498-1-Ig)

    WB analysis of NIH/3T3 using 67498-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using AKR1B1 Monoclonal antibody (67498-1-Ig)

    WB analysis of 4T1 using 67498-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67498-1-Ig targets AKR1B1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AKR1B1 fusion protein Ag7571 相同性解析による交差性が予測される生物種
Full Name aldo-keto reductase family 1, member B1 (aldose reductase)
Calculated molecular weight 36 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC000260
Gene symbol AKR1B1
Gene ID (NCBI) 231
RRIDAB_2882722
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AKR1B1 antibody 67498-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

An Advanced Systems Pharmacology Strategy Reveals AKR1B1, MMP2, PTGER3 as Key Genes in the Competing Endogenous RNA Network of Compound Kushen Injection Treating Gastric Carcinoma by Integrated Bioinformatics and Experimental Verification

Authors - Wei Zhou
View Article
mouseWB

Pharmacol Res

Inulin-type fructans change the gut microbiota and prevent the development of diabetic nephropathy.

Authors - Liman Luo
View Article