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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AKR1B10 Monoclonal antibody

AKR1B10 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5A4G5

Cat no : 68327-1-Ig

Synonyms

AKR1B10, AKR1B11, AKR1B12, Aldose reductase like, ALDRLn, ARL 1, ARL1, ARP, hARP, HIS, HSI, SI reductase, Small intestine reductase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKR1B10 Monoclonal antibody (68327-1-Ig)

    WB analysis using 68327-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat stomach tissue using AKR1B10 Monoclonal antibody (68327-1-Ig)

    WB analysis of rat stomach using 68327-1-Ig

    rat stomach tissue were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig small intestine tissue using AKR1B10 Monoclonal antibody (68327-1-Ig)

    WB analysis of pig small intestine using 68327-1-Ig

    pig small intestine tissue were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using AKR1B10 Monoclonal antibody (68327-1-Ig)

    IF Staining of A549 using 68327-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using AKR1B10 antibody (68327-1-Ig, Clone: 5A4G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, rat stomach tissue, pig small intestine tissue, A549 cells, pig stomach tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68327-1-Ig targets AKR1B10 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Pig, Rat samples.

Tested Reactivity Human, Pig, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AKR1B10 fusion protein Ag24406 相同性解析による交差性が予測される生物種
Full Name aldo-keto reductase family 1, member B10 (aldose reductase)
Calculated molecular weight 316 aa, 36 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC008837
Gene symbol AKR1B10
Gene ID (NCBI) 57016
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aldo-keto reductase family 1 member B10 (AKR1B10), which belongs to the AKR superfamily, consists of 316 amino acids and its gene is located on chromosome 7q33. AKR1B10 was initially identified in liver cancer. It is highly expressed in the small intestine, colon, and adrenal gland.

Protocols

Product Specific Protocols
WB protocol for AKR1B10 antibody 68327-1-IgDownload protocol
IF protocol for AKR1B10 antibody 68327-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols