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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using AKR1CL2 Polyclonal antibody (20525-1-AP)

    WB analysis of mouse testis using 20525-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using AKR1CL2 Polyclonal antibody (20525-1-AP)

    WB analysis of RAW264.7 using 20525-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using AKR1CL2 Polyclonal antibody (20525-1-AP)

    WB analysis of HT-1080 using 20525-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AKR1CL2 Polyclonal antibody (20525-1-AP)

    IF Staining of HeLa using 20525-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AKR1CL2 antibody (20525-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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AKR1CL2 Polyclonal antibody

AKR1CL2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20525-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Aldo-keto reductase family 1 member C-like protein 2, AKRDC1, AKR1E2, AKR1CL 2, AF reductase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, HT-1080 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20525-1-AP targets AKR1CL2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKR1CL2 fusion protein Ag14381 相同性解析による交差性が予測される生物種
Full Name aldo-keto reductase family 1, member C-like 2
Calculated molecular weight 320 aa, 37 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002862
Gene Symbol AKR1CL2
Gene ID (NCBI) 83592
RRIDAB_10694285
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JD6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AKR1CL2 antibody 20525-1-APDownload protocol
IF protocol for AKR1CL2 antibody 20525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols