Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23910-1-AP targets AKR1D1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AKR1D1 fusion protein Ag21009 相同性解析による交差性が予測される生物種 |
| Full Name | aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) |
| Calculated molecular weight | 326 aa, 37 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC130627 |
| Gene Symbol | AKR1D1 |
| Gene ID (NCBI) | 6718 |
| RRID | AB_2879359 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P51857 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AKR1D1 (steroid 5β-reductase) reduces all Δ 4-3-ketosteroids to form 5β-dihydrosteroids, a first step in the clearance of steroid hormones and an essential step in the synthesis of all bile acids. It is also named as SRD5B1 and belongs to the aldo/keto reductase family. This protein has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AKR1D1 antibody 23910-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |