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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using AKR7A3 Polyclonal antibody (13209-1-AP)

    WB analysis of human liver using 13209-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using AKR7A3 Polyclonal antibody (13209-1-AP)

    WB analysis of human brain using 13209-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AKR7A3 Polyclonal antibody (13209-1-AP)

    WB analysis of HepG2 using 13209-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using AKR7A3 Polyclonal antibody (13209-1-AP)

    WB analysis of NIH/3T3 using 13209-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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AKR7A3 Polyclonal antibody

AKR7A3 Polyclonal Antibody for WB, ELISA
Cat No. 13209-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EC:1.-.-.-, AFB1-AR 2, AFB1 AR 2, AFB1 aldehyde reductase 2, AFAR2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman liver tissue, HepG2 cells, human brain tissue, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13209-1-AP targets AKR7A3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKR7A3 fusion protein Ag3991 相同性解析による交差性が予測される生物種
Full Name aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase)
Calculated molecular weight 331 aa, 37 kDa
Observed molecular weight 37 kDa and 55-60 kDa
GenBank accession numberBC025709
Gene Symbol AKR7A3
Gene ID (NCBI) 22977
RRIDAB_2224549
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95154
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKR7A3 belongs to the aldo‐keto reductase (AKR) superfamily, whose primary role is to reduce aldehydes and ketones to generate primary and secondary alcohols, respectively. These enzymes have been shown to play crucial roles in drug metabolism, carcinogen metabolism, and cellular metabolism. Growing evidence suggests that AKR7A3 can play an essential role in the occurrence of cancers, including breast and liver cancers. (PMID: 36951402)

Protocols

Product Specific Protocols
WB protocol for AKR7A3 antibody 13209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Toxicol Sci

Generation of A New Model Rat: Nrf2 Knockout Rats Are Sensitive to Aflatoxin B1 Toxicity.

Authors - Keiko Taguchi
View Article