Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis of MCF-7 using 10176-2-AP
WB result of AKT antibody (10176-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected MCF-7 cells.
WB analysis using 10176-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 10176-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa cells using 10176-2-AP
siRNA AKT1 result from Dr. Eva Martinez-Balibrea. Green:tubulin, Red:10176-2-AP, AKT1.
WB analysis using 10176-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10176-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:500.
WB analysis using 10176-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10176-2-AP
IP result of anti-AKT (IP:10176-2-AP, 4ug; Detection:10176-2-AP 1:15000) with HeLa cells lysate 1085 ug.
IHC staining of human ovary tumor using 10176-2-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10176-2-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10176-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10176-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 10176-2-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AKT antibody (10176-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

AKT Polyclonal antibody

AKT Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 10176-2-AP

Publications(1359)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Synonyms

AKT pan, AKT 1, AKT (pan), AKT1/2/3, AKT1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1359)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse liver tissue, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary tumor tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 1328 publications below
IHC	See 37 publications below
IF	See 20 publications below
IP	See 8 publications below
ELISA	See 1 publications below
CoIP	See 3 publications below

Product Information

10176-2-AP targets AKT in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey, chicken, zebrafish, sheep, goat, fish, zebra finches
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AKT fusion protein Ag0213 相同性解析による交差性が予測される生物種
Full Name	v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight	56 kDa
Observed molecular weight	56-62 kDa
GenBank accession number	BC000479
Gene Symbol	AKT1
Gene ID (NCBI)	207
RRID	AB_2224574
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P31749
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Protocols

Product Specific Protocols
WB protocol for AKT antibody 10176-2-AP	Download protocol
IHC protocol for AKT antibody 10176-2-AP	Download protocol
IF protocol for AKT antibody 10176-2-AP	Download protocol
IP protocol for AKT antibody 10176-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res RAB31 marks and controls an ESCRT-independent exosome pathway. Authors - Denghui Wei View Article
mouse	WB	Nat Cell Biol Heat stress activates YAP/TAZ to induce the heat shock transcriptome. Authors - Min Luo View Article
human	WB	Nat Commun Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk Authors - Pingting Ying View Article
human	WB	Adv Sci (Weinh) Cis-Regulation of an m6A Eraser by an Insertion Variant Associated with Survival of Patients With Non-Small Cell Lung Carcinoma Authors - Lei Cheng View Article
human	WB	Sci Adv Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes. Authors - Patrick Han View Article
human,mouse	WB	Acta Pharm Sin B Dissecting the novel abilities of aripiprazole: The generation of anti-colorectal cancer effects by targeting Gαq via HTR2B Authors - Haowei Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MCF-7 using 10176-2-AP

WB analysis using 10176-2-AP

WB analysis using 10176-2-AP

WB analysis of HeLa cells using 10176-2-AP

WB analysis using 10176-2-AP

WB analysis of A549 using 10176-2-AP

WB analysis using 10176-2-AP

IP experiment of HeLa using 10176-2-AP

IHC staining of human ovary tumor using 10176-2-AP

IHC staining of human ovary tumor using 10176-2-AP

IHC staining of human breast cancer using 10176-2-AP

IHC staining of human breast cancer using 10176-2-AP

IF Staining of mouse brain using 10176-2-AP

AKT Polyclonal antibody

AKT Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

RAB31 marks and controls an ESCRT-independent exosome pathway.

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk

Cis-Regulation of an m6A Eraser by an Insertion Variant Associated with Survival of Patients With Non-Small Cell Lung Carcinoma

Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.

Dissecting the novel abilities of aripiprazole: The generation of anti-colorectal cancer effects by targeting Gαq via HTR2B