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AKT Polyclonal antibody
AKT Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IHC, IF-P, IP, CoIP, ELISA
Conjugate
Unconjugated
1246
Cat no : 10176-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse liver tissue, rat brain tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 8 publications below |
WB | See 1217 publications below |
IHC | See 35 publications below |
IF | See 19 publications below |
IP | See 8 publications below |
ELISA | See 1 publications below |
CoIP | See 3 publications below |
Product Information
10176-2-AP targets AKT in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, zebrafish, hamster, sheep, goat, fish, zebra finches |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AKT fusion protein Ag0213 相同性解析による交差性が予測される生物種 |
Full Name | v-akt murine thymoma viral oncogene homolog 1 |
Calculated molecular weight | 56 kDa |
Observed molecular weight | 56-62 kDa |
GenBank accession number | BC000479 |
Gene symbol | AKT1 |
Gene ID (NCBI) | 207 |
RRID | AB_2224574 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for AKT antibody 10176-2-AP | Download protocol |
IHC protocol for AKT antibody 10176-2-AP | Download protocol |
IF protocol for AKT antibody 10176-2-AP | Download protocol |
IP protocol for AKT antibody 10176-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk | ||
Sci Adv Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes. | ||
Acta Pharm Sin B Dissecting the novel abilities of aripiprazole: The generation of anti-colorectal cancer effects by targeting Gαq via HTR2B | ||
Acta Pharm Sin B Oral nano-formulation improves pancreatic islets dysfunction via lymphatic transport for antidiabetic treatment |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Hyopil (Verified Customer) (08-23-2021) | Worked well with western blotting
|
FH Azita (Verified Customer) (06-02-2021) | Western blot analysis using AKT polyclonal antibody in NSC34 cell line at dilution of 1:500.
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