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- KD/KO Validated
AKT1/3 Monoclonal antibody
AKT1/3 Monoclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
3G5E11
Cat no : 60203-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, MCF-7 cells, rat liver tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 52 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 60203-1-Ig is AKT1/3 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, huamn |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AKT1/3 fusion protein Ag16695 相同性解析による交差性が予測される生物種 |
Full Name | v-akt murine thymoma viral oncogene homolog 1 |
Calculated molecular weight | 56 kDa |
Observed molecular weight | 56-62 kDa |
GenBank accession number | BC000479 |
Gene symbol | AKT1 |
Gene ID (NCBI) | 207 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The serine-threonine protein kinase AKT is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT, which then phosphorylates and inactivates components of the apoptotic machinery.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for AKT1/3 antibody 60203-1-Ig | Download protocol |
IHC protocol for AKT1/3 antibody 60203-1-Ig | Download protocol |
IF protocol for AKT1/3 antibody 60203-1-Ig | Download protocol |
IP protocol for AKT1/3 antibody 60203-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Exp Clin Cancer Res Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization | ||
Sci Total Environ Effects of triclosan exposure on stem cells from human exfoliated deciduous teeth (SHED) fate | ||
Autophagy BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation. | ||
Cancer Lett Endogenous production of C-C motif chemokine ligand 2 by nasopharyngeal carcinoma cells drives radioresistance-associated metastasis. | ||
Cell Death Dis Elaiophylin reduces body weight and lowers glucose levels in obese mice by activating AMPK |