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  • Featured Product
  • KD/KO Validated

AKT Monoclonal antibody, PBS Only (Detector)

AKT Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

2C5D1

Cat no : 60203-2-PBS

Synonyms

AKT, AKT (pan), AKT pan, AKT1, AKT1/2/3, pan AKT, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha, total AKT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis using 60203-2-Ig (same clone as 60203-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of A549 using 60203-2-Ig (same clone as 60203-2-PBS)

    WB result of AKT antibody (60203-2-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected A549 cells. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of HEK-293 using 60203-2-Ig (same clone as 60203-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) and 66444-1-Ig(AKT1-phospho-S473 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of HEK-293 using 60203-2-Ig (same clone as 60203-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of NIH/3T3 using 60203-2-Ig (same clone as 60203-2-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of RAW 264.7 using 60203-2-Ig (same clone as 60203-2-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of mouse brain using 60203-2-Ig (same clone as 60203-2-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of ROS1728 using 60203-2-Ig (same clone as 60203-2-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of rat liver using 60203-2-Ig (same clone as 60203-2-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using AKT Monoclonal antibody (60203-2-Ig)

    IP experiment of mouse brain using 60203-2-Ig (same clone as 60203-2-PBS)

    IP result of anti-AKT (IP:60203-2-Ig, 5ug; Detection:60203-2-Ig 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human cervical cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human cervical cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AKT Monoclonal antibody (60203-2-Ig)

    IF Staining of MCF-7 using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AKT antibody (60203-2-Ig, Clone: 2C5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP50177-1

    Cytometric bead array in cell lysate using MP50177-1

    Cytometric bead array in cell lysate using MP50177-1, Phospho-AKT(Ser473) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66444-1-PBS. Detection antibody: 60203-2-PBS. Cell lysate: Non-treated HEK293 and Calyculin A treated HEK293(30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60203-2-PBS targets AKT as part of a matched antibody pair:

MP50177-1: 66444-1-PBS capture and 60203-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AKT fusion protein Ag16695 相同性解析による交差性が予測される生物種
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight 56 kDa
Observed molecular weight 56-62 kDa
GenBank accession numberBC000479
Gene symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.