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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKT1-Specific Recombinant antibody (80816-1-RR)

    WB analysis using 80816-1-RR (same clone as 80816-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80816-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using AKT1-Specific Recombinant antibody (80816-1-RR)

    WB analysis of MCF-7 using 80816-1-RR (same clone as 80816-1-PBS)

    WB result of AKT1 antibody (80816-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using AKT1-Specific Recombinant antibody (80816-1-RR)

    IP experiment of HEK-293 using 80816-1-RR (same clone as 80816-1-PBS)

    IP result of anti-AKT1 (IP:80816-1-RR, 4ug; Detection:80816-1-RR 1:2000) with HEK-293 cells lysate 1280 ug. This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AKT1-Specific Recombinant antibody (80816-1-RR)

    IHC staining of human ovary tumor using 80816-1-RR (same clone as 80816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AKT1-Specific Recombinant antibody (80816-1-RR)

    IHC staining of human ovary tumor using 80816-1-RR (same clone as 80816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using AKT1-Specific Recombinant antibody (80816-1-RR)

    FC experiment of Jurkat using 80816-1-RR (same clone as 80816-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human AKT1 (80816-1-RR, Clone:5O9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug control antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

AKT1-Specific Recombinant antibody, PBS Only

AKT1-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 80816-1-PBS
Clone No.5O9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

AKT pan, AKT 1, AKT (pan), AKT, AKT1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80816-1-PBS targets AKT1-Specific in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AKT1-Specific fusion protein Ag0213 相同性解析による交差性が予測される生物種
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight 56 kDa
Observed molecular weight 56-62 kDa
GenBank accession numberBC000479
Gene Symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31749
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. 80816-1-RR recognizes AKT1 specifically.