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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AKT1 Recombinant antibody, PBS Only

AKT1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4I5

Cat no : 80457-1-PBS

Synonyms

AKT1 (C-terminal), AKT pan, AKT 1, AKT (pan), AKT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKT1 Recombinant antibody (80457-1-RR)

    WB analysis using 80457-1-RR (same clone as 80457-1-PBS)

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80457-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80457-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AKT1 Recombinant antibody (80457-1-RR)

    WB analysis using 80457-1-RR (same clone as 80457-1-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80457-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80457-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using AKT1 Recombinant antibody (80457-1-RR)

    WB analysis of HepG2 using 80457-1-RR (same clone as 80457-1-PBS)

    Calyculin A treated HepG2 cells were subjected to SDS PAGE followed by western blot with 80457-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80457-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using AKT1 Recombinant antibody (80457-1-RR)

    WB analysis of HEK-293T using 80457-1-RR (same clone as 80457-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 80457-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT1 Recombinant antibody (80457-1-RR)

    IHC staining of human breast cancer using 80457-1-RR (same clone as 80457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80457-1-RR (AKT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT1 Recombinant antibody (80457-1-RR)

    IHC staining of human breast cancer using 80457-1-RR (same clone as 80457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80457-1-RR (AKT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80457-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AKT1 Recombinant antibody (80457-1-RR)

    IF Staining of HeLa using 80457-1-RR (same clone as 80457-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AKT1 (C-terminal) antibody (80457-1-RR, Clone: 4I5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80457-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80457-1-PBS targets AKT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name v-akt murine thymoma viral oncogene homolog 1
Observed molecular weight56-62 kDa
GenBank accession numberNM_005163
Gene symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672). 80457-1-RR specifically recognizes AKT1.