ALAS1 Polyclonal antibody

ALAS1 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 16200-1-AP

Synonyms

ALAS H, ALAS 3, Alas 1, ALAS, 5-aminolevulinic acid synthase 1


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Tested Applications

Positive WB detected inHeLa cells, Raji cells
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16200-1-AP targets ALAS1 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALAS1 fusion protein Ag9126 相同性解析による交差性が予測される生物種
Full Name aminolevulinate, delta-, synthase 1
Calculated molecular weight 640 aa, 71 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC011798
Gene symbol ALAS1
Gene ID (NCBI) 211
RRIDAB_2878227
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ALAS1 antibody 16200-1-APDownload protocol
IF protocol for ALAS1 antibody 16200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

Authors - Ying Wang
humanWB

Mol Cell

Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability.

Authors - Gokhan Unlu
ratWB

Naunyn Schmiedebergs Arch Pharmacol

Role of aminolevulinic acid synthase 1 in doxorubicin-induced oxidative stress to the ardiomyocyte.

Authors - Zuoyan Wang
humanIF

Saudi J Gastroenterol

Inhibition of ALAS1 activity exerts anti-tumour effects on colorectal cancer in vitro.

Authors - Yalei Zhao
humanWB

Evid Based Complement Alternat Med

Genetic and Functional Evaluation of the Role of FOXO1 in Antituberculosis Drug-Induced Hepatotoxicity.

Authors - Jingwei Zhang
humanWB

Arq Bras Cardiol

Effects of Doxorubicin on Heme Biosynthesis and Metabolism in Cardiomyocyte.

Authors - Zuoyan Wang