Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Raji cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IF | See 1 publications below |
Product Information
16200-1-AP targets ALAS1 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ALAS1 fusion protein Ag9126 相同性解析による交差性が予測される生物種 |
| Full Name | aminolevulinate, delta-, synthase 1 |
| Calculated molecular weight | 640 aa, 71 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC011798 |
| Gene Symbol | ALAS1 |
| Gene ID (NCBI) | 211 |
| RRID | AB_2878227 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13196 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALAS1 antibody 16200-1-AP | Download protocol |
| IF protocol for ALAS1 antibody 16200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability. | ||
Naunyn Schmiedebergs Arch Pharmacol Role of aminolevulinic acid synthase 1 in doxorubicin-induced oxidative stress to the ardiomyocyte. | ||
Saudi J Gastroenterol Inhibition of ALAS1 activity exerts anti-tumour effects on colorectal cancer in vitro. | ||
Evid Based Complement Alternat Med Genetic and Functional Evaluation of the Role of FOXO1 in Antituberculosis Drug-Induced Hepatotoxicity. | ||
Arq Bras Cardiol Effects of Doxorubicin on Heme Biosynthesis and Metabolism in Cardiomyocyte. |