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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALAS2 Polyclonal antibody

ALAS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30539-1-AP

Synonyms

ALAS E, ALAS2, ALASE, ANH1, ASB, Delta ALA synthase 2, FLJ93603, XLSA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALAS2 Polyclonal antibody (30539-1-AP)

    WB analysis using 30539-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30539-1-AP (ALAS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inK-562 cells, NIH/3T3 cells, mouse brain tissue, mouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30539-1-AP targets ALAS2 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALAS2 fusion protein Ag30308 相同性解析による交差性が予測される生物種
Full Name aminolevulinate, delta-, synthase 2
Calculated molecular weight 587 aa, 65 kDa
Observed molecular weight65 kDa
GenBank accession numberBC030230
Gene symbol ALAS2
Gene ID (NCBI) 212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.

Protocols

Product Specific Protocols
WB protocol for ALAS2 antibody 30539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols