Validation Data Gallery
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Tested Applications
| Positive WB detected in | THP-1 cells, HEK-293 cells, Daudi cells, NK-92 cells, Raji cells, MOLT-4 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68332-1-Ig targets ALAS2 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ALAS2 fusion protein Ag13524 相同性解析による交差性が予測される生物種 |
| Full Name | aminolevulinate, delta-, synthase 2 |
| Calculated molecular weight | 587 aa, 65 kDa |
| Observed molecular weight | 60~65 kDa |
| GenBank accession number | BC030230 |
| Gene Symbol | ALAS2 |
| Gene ID (NCBI) | 212 |
| RRID | AB_3085057 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P22557 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALAS2 antibody 68332-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |