• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALAS2 Monoclonal antibody

ALAS2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C1H1

Cat no : 68332-1-Ig

Synonyms

ALAS E, ALAS2, ALASE, ANH1, ASB, Delta ALA synthase 2, FLJ93603, XLSA

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ALAS2 Monoclonal antibody (68332-1-Ig)

    WB analysis using 68332-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ALAS2 Monoclonal antibody (68332-1-Ig)

    WB analysis using 68332-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ALAS2 Monoclonal antibody (68332-1-Ig)

    WB analysis using 68332-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NK-92 cells using ALAS2 Monoclonal antibody (68332-1-Ig)

    WB analysis of NK-92 using 68332-1-Ig

    NK-92 cells were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inTHP-1 cells, HEK-293 cells, Daudi cells, NK-92 cells, Raji cells, MOLT-4 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68332-1-Ig targets ALAS2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALAS2 fusion protein Ag13524 相同性解析による交差性が予測される生物種
Full Name aminolevulinate, delta-, synthase 2
Calculated molecular weight 587 aa, 65 kDa
Observed molecular weight60~65 kDa
GenBank accession numberBC030230
Gene symbol ALAS2
Gene ID (NCBI) 212
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.

Protocols

Product Specific Protocols
WB protocol for ALAS2 antibody 68332-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols