Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICC

WB analysis of human plasma using 16475-1-AP
human blood were subjected to SDS PAGE followed by western blot with 16475-1-AP (ALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16475-1-AP
WB result of Albumin antibody (16475-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Albumin transfected HepG2 cells.
WB analysis using 16475-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16475-1-AP (Albumin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse serum using 16475-1-AP
mouse serum were subjected to SDS PAGE followed by western blot with 16475-1-AP (Albumin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP experiment of human plasma using 16475-1-AP
IP result of anti-Albumin (IP:16475-1-AP, 4ug; Detection:16475-1-AP 1:10000) with human plasma lysate 4000ug.
IHC staining of human liver using 16475-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 16475-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon using 16475-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16475-1-AP (Albumin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 16475-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 16475-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse liver using 16475-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse liver using 16475-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse liver using 16475-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of L02 using 16475-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using Albumin antibody (16475-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

Albumin Polyclonal antibody

Albumin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 16475-1-AP

Publications(158)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Synonyms

ALB, Serum albumin, HSA

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (158)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human blood tissue, human plasma, mouse serum, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	human plasma tissue
Positive IHC detected in	human liver tissue, mouse liver tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse liver tissue
Positive IF/ICC detected in	L02 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 92 publications below
IHC	See 14 publications below
IF	See 60 publications below
ELISA	See 1 publications below
CoIP	See 1 publications below

Product Information

16475-1-AP targets Albumin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Albumin fusion protein Ag9569 相同性解析による交差性が予測される生物種
Full Name	albumin
Calculated molecular weight	609 aa, 69 kDa
Observed molecular weight	66 kDa
GenBank accession number	BC034023
Gene Symbol	Albumin
Gene ID (NCBI)	213
RRID	AB_2242567
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P02768
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. In additon, glycated serum albumin is also a potential diabetes biomarker. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)

Protocols

Product Specific Protocols
WB protocol for Albumin antibody 16475-1-AP	Download protocol
IHC protocol for Albumin antibody 16475-1-AP	Download protocol
IF protocol for Albumin antibody 16475-1-AP	Download protocol
IP protocol for Albumin antibody 16475-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Science Enterically derived high-density lipoprotein restrains liver injury through the portal vein. Authors - Yong-Hyun Han View Article
mouse	IF	Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration Authors - Jinpeng Du View Article
human, mouse	WB,IF	Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. Authors - Li Jianhua J View Article
human	IHC	Immunity The zinc finger protein Miz1 suppresses liver tumorigenesis by restricting hepatocyte-driven macrophage activation and inflammation. Authors - Wenjie Zhang View Article
mouse	WB	Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis Authors - Xiao Wang View Article
human	WB	Cell Res In vivo self-assembled small RNAs as a new generation of RNAi therapeutics. Authors - Zheng Fu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human plasma using 16475-1-AP

WB analysis of HepG2 using 16475-1-AP

WB analysis using 16475-1-AP

WB analysis of mouse serum using 16475-1-AP

IP experiment of human plasma using 16475-1-AP

IHC staining of human liver using 16475-1-AP

IHC staining of human liver using 16475-1-AP

IHC staining of human colon using 16475-1-AP

IHC staining of mouse liver using 16475-1-AP

IHC staining of mouse liver using 16475-1-AP

IF Staining of mouse liver using 16475-1-AP

IF Staining of mouse liver using 16475-1-AP

IF Staining of mouse liver using 16475-1-AP

IF Staining of L02 using 16475-1-AP

Albumin Polyclonal antibody

Albumin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Enterically derived high-density lipoprotein restrains liver injury through the portal vein.

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

The zinc finger protein Miz1 suppresses liver tumorigenesis by restricting hepatocyte-driven macrophage activation and inflammation.

Manganese regulation of COPII condensation controls circulating lipid homeostasis

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.