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Albumin Polyclonal antibody
Albumin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA
Conjugate
Unconjugated
142
Cat no : 16475-1-AP
Synonyms
"Albumin Antibodies" Comparison
View side-by-side comparison of Albumin antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | human blood tissue, human plasma, mouse serum, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | human plasma tissue |
| Positive IHC detected in | human liver tissue, mouse liver tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 79 publications below |
| IHC | See 14 publications below |
| IF | See 57 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
16475-1-AP targets Albumin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Albumin fusion protein Ag9569 相同性解析による交差性が予測される生物種 |
| Full Name | albumin |
| Calculated molecular weight | 609 aa, 69 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC034023 |
| Gene symbol | Albumin |
| Gene ID (NCBI) | 213 |
| RRID | AB_2242567 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. In additon, glycated serum albumin is also a potential diabetes biomarker. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Albumin antibody 16475-1-AP | Download protocol |
| IHC protocol for Albumin antibody 16475-1-AP | Download protocol |
| IF protocol for Albumin antibody 16475-1-AP | Download protocol |
| IP protocol for Albumin antibody 16475-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Enterically derived high-density lipoprotein restrains liver injury through the portal vein. | ||
Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration | ||
Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. | ||
Immunity The zinc finger protein Miz1 suppresses liver tumorigenesis by restricting hepatocyte-driven macrophage activation and inflammation. | ||
Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kamal (Verified Customer) (01-26-2024) | For western blot analysis, antibody worked well at dilution of 1:25000 in 1 X TBST. For immunofluorescence, antibody worked well at dilution of 1:200 in 1 X PBS.
|
FH Kamal (Verified Customer) (01-26-2024) | Liver tissues were well stained in provided dilution with 1X PBS.
|
