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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALCAM Monoclonal antibody (67768-1-Ig)

    WB analysis using 67768-1-Ig (same clone as 67768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67768-1-Ig (ALCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ALCAM Monoclonal antibody (67768-1-Ig)

    IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ALCAM Monoclonal antibody (67768-1-Ig)

    IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using ALCAM Monoclonal antibody (67768-1-Ig)

    IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using ALCAM Monoclonal antibody (67768-1-Ig)

    IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse breast cancer using ALCAM Monoclonal antibody (67768-1-Ig)

    IF Staining of mouse breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using ALCAM Monoclonal antibody (67768-1-Ig)

    IF Staining of hTERT-RPE1 using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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ALCAM Monoclonal antibody, PBS Only

ALCAM Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA
Cat No. 67768-1-PBS
Clone No.1F7D6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Synonyms

MEMD, CD166, 1F7D6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67768-1-PBS targets ALCAM in WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALCAM fusion protein Ag16892 相同性解析による交差性が予測される生物種
Full Name activated leukocyte cell adhesion molecule
Calculated molecular weight 583 aa, 65 kDa
Observed molecular weight90-100 kDa
GenBank accession numberBC137097
Gene Symbol ALCAM
Gene ID (NCBI) 214
RRIDAB_2918534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13740
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).