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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALCAM Recombinant antibody

ALCAM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241945A4

Cat no : 84528-1-RR

Synonyms

Protein DM-GRASP, CD166 antigen, CD166, BEN, Activated leukocyte cell adhesion molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using ALCAM Recombinant antibody (84528-1-RR)

    WB analysis of mouse lung using 84528-1-RR

    mouse lung tissue was subjected to SDS PAGE followed by western blot with 84528-1-RR (ALCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ALCAM Recombinant antibody (84528-1-RR)

    IHC staining of mouse liver using 84528-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ALCAM Recombinant antibody (84528-1-RR)

    IHC staining of mouse liver using 84528-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84528-1-RR targets ALCAM in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name activated leukocyte cell adhesion molecule
Calculated molecular weight65kDa
Observed molecular weight90-100 kDa
GenBank accession numberNM_009655.3
Gene symbol Alcam
Gene ID (NCBI) 11658
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

Protocols

Product Specific Protocols
WB protocol for ALCAM antibody 84528-1-RRDownload protocol
IHC protocol for ALCAM antibody 84528-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols