Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICCFC (Intra)

WB analysis using 15910-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 15910-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 15910-1-AP
WB result of ALDH1A1 antibody (15910-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH1A1 transfected K-562 cells.
WB analysis using 15910-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 15910-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 15910-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 15910-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 15910-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 15910-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse colon using 15910-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 15910-1-AP (ALDH1A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse colon using 15910-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 15910-1-AP (ALDH1A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse testis using 15910-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A549 using 15910-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ALDH1A1 antibody (15910-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HepG2 using 15910-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ALDH1A1 (15910-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

ALDH1A1 Polyclonal antibody

ALDH1A1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Cat No. 15910-1-AP

Publications(137)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA

Synonyms

ALDH1A 1, ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (137)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, mouse kidney tissue
Positive IHC detected in	mouse testis tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse colon tissue, mouse testis tissue
Positive IF/ICC detected in	A549 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 115 publications below
IHC	See 25 publications below
IF	See 16 publications below
ELISA	See 2 publications below
CoIP	See 1 publications below

Product Information

15910-1-AP targets ALDH1A1 in WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ALDH1A1 fusion protein Ag8665 相同性解析による交差性が予測される生物種
Full Name	aldehyde dehydrogenase 1 family, member A1
Calculated molecular weight	501 aa, 55 kDa
Observed molecular weight	50-55 kDa
GenBank accession number	BC001505
Gene Symbol	ALDH1A1
Gene ID (NCBI)	216
RRID	AB_2305276
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P00352
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family.

Protocols

Product Specific Protocols
WB protocol for ALDH1A1 antibody 15910-1-AP	Download protocol
IHC protocol for ALDH1A1 antibody 15910-1-AP	Download protocol
IF protocol for ALDH1A1 antibody 15910-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders. Authors - Xingxing Xu View Article
mouse	WB	Mol Cell Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome. Authors - Nina Oberbeck View Article
human	WB,IHC,IF	Int J Oral Sci LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma Authors - Yuantong Liu View Article
human	WB,IHC	Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. Authors - Rongxuan Zhu View Article
human,mouse	WB,IHC,IF	J Pineal Res Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis Authors - Xinyue Luo View Article
human	IF	EMBO J RASSF1C oncogene elicits amoeboid invasion, cancer stemness, and extracellular vesicle release via a SRC/Rho axis. Authors - Maria Laura Tognoli View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15910-1-AP

WB analysis using 15910-1-AP

WB analysis of K-562 using 15910-1-AP

WB analysis using 15910-1-AP

IHC staining of mouse testis using 15910-1-AP

IHC staining of mouse testis using 15910-1-AP

IHC staining of human colon using 15910-1-AP

IHC staining of human liver using 15910-1-AP

IHC staining of human liver using 15910-1-AP

IF Staining of mouse colon using 15910-1-AP

IF Staining of mouse colon using 15910-1-AP

IF Staining of mouse testis using 15910-1-AP

IF Staining of A549 using 15910-1-AP

FC experiment of HepG2 using 15910-1-AP

ALDH1A1 Polyclonal antibody

ALDH1A1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders.

Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.

LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis

RASSF1C oncogene elicits amoeboid invasion, cancer stemness, and extracellular vesicle release via a SRC/Rho axis.