ALDH1A2 Polyclonal antibody

ALDH1A2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13951-1-AP

Synonyms

ALDH1A2, RALDH 2, RALDH(II), RALDH2, RALDH2 T, Retinal dehydrogenase 2


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Tested Applications

Positive WB detected inK-562 cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13951-1-AP targets ALDH1A2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1A2 fusion protein Ag5062 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 1 family, member A2
Calculated molecular weight 518 aa, 57 kDa
Observed molecular weight 53-57 kDa
GenBank accession numberBC030589
Gene symbol ALDH1A2
Gene ID (NCBI) 8854
RRIDAB_2224033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALDH1A2(aldehyde dehydrogenase family 1 member A2) is also named as RALDH2(retinal dehydrogenase 2) and belongs to the aldehyde dehydrogenase family. It is a cytosolic homotetramer(56.7 kDa subunits) exhibiting complex expression patterns throughout embryonic development. ALDH1A2 plays a role in retinoid metabolism during embryonic development where it is considered to be the major retinoic acid-synthesizing enzyme during early embryogenesis(PMID:20735668). Its expression is reduced in primary prostate tumors compared with normal prostate tissue suggested that it is normally expressed in the epithelial compartment of prostate tissue(PMID:16166285).It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ALDH1A2 antibody 13951-1-APDownload protocol
IHC protocol for ALDH1A2 antibody 13951-1-APDownload protocol
IP protocol for ALDH1A2 antibody 13951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
humanWB

Elife

FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.

Authors - Carlos J Diaz Osterman
humanWB

iScience

Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression

Authors - Zhilei Zhang
mouseWB

J Nutr Biochem

All-trans retinoic acid induces lipophagy through the activation of the AMPK-Beclin1 signaling pathway and reduces Rubicon expression in adipocytes

Authors - Yuki Mori
humanWB

Am J Cancer Res

Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression

Authors - Bing-Yen Wang
humanIHC

J Lipid Res

Importance of ALDH1A enzymes in determining human testicular retinoic acid concentrations.

Authors - Samuel L Arnold