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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALDH1A3 Polyclonal antibody

ALDH1A3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29373-1-AP

Synonyms

Aldehyde dehydrogenase 6, ALDH1A3, ALDH1A6, ALDH6, RALDH 3, RALDH3, Retinaldehyde dehydrogenase 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDH1A3 Polyclonal antibody (29373-1-AP)

    WB analysis using 29373-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29373-1-AP (ALDH1A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALDH1A3 Polyclonal antibody (29373-1-AP)

    IHC staining of human liver cancer using 29373-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29373-1-AP (ALDH1A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALDH1A3 Polyclonal antibody (29373-1-AP)

    IHC staining of human liver cancer using 29373-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29373-1-AP (ALDH1A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29373-1-AP targets ALDH1A3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1A3 fusion protein Ag31100 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 1 family, member A3
Calculated molecular weight 512 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC069274
Gene symbol ALDH1A3
Gene ID (NCBI) 220
RRIDAB_2918287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ALDH1A3 is the predominant ALDH isozyme that participates in various physiological processes in human cells. Growing evidence has shown that ALDH1A3 exhibits high activity in cancer stem cells (CSCs) and influences a diverse range of biological characteristics within these cells. (PMID: 30958800,PMID: 32612951)

Protocols

Product Specific Protocols
WB protocol for ALDH1A3 antibody 29373-1-APDownload protocol
IHC protocol for ALDH1A3 antibody 29373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Exp Clin Cancer Res

Tumor associated macrophages-derived exosomes facilitate hepatocellular carcinoma malignance by transferring lncMMPA to tumor cells and activating glycolysis pathway

Authors - Minghao Xu
View Article