ALDH1B1 Polyclonal antibody

Name: ALDH1B1 Polyclonal antibody
Brand: Proteintech
SKU: 15560-1-AP

ALDH1B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15560-1-AP

Synonyms

EC:1.2.1.3, ALDHX, ALDH5, Aldehyde dehydrogenase X, mitochondrial, Aldehyde dehydrogenase 5

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HepG2 using 15560-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 15560-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15560-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15560-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15560-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 15560-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15560-1-AP
IP result of anti-ALDH1B1 (IP:15560-1-AP, 4ug; Detection:15560-1-AP 1:20000) with mouse brain tissue lysate 2100 ug.
IHC staining of human testis using 15560-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 15560-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 15560-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ALDH1B1 antibody (15560-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

-/ -

Cat No. 15560-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, K-562 cells, mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 12 publications below
IHC	See 5 publications below

Product Information

15560-1-AP targets ALDH1B1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ALDH1B1 fusion protein Ag7144 相同性解析による交差性が予測される生物種
Full Name	aldehyde dehydrogenase 1 family, member B1
Calculated molecular weight	57 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC001619
Gene symbol	ALDH1B1
Gene ID (NCBI)	219
RRID	AB_2224162
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112).

Protocols

Product Specific Protocols
WB protocol for ALDH1B1 antibody 15560-1-AP	Download protocol
IHC protocol for ALDH1B1 antibody 15560-1-AP	Download protocol
IF protocol for ALDH1B1 antibody 15560-1-AP	Download protocol
IP protocol for ALDH1B1 antibody 15560-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Cell Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome. Authors - Nina Oberbeck View Article
human	WB	Elife FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy. Authors - Carlos J Diaz Osterman View Article
mouse	IHC	J Transl Med Identification and experimental validation of KMO as a critical immune-associated mitochondrial gene in unstable atherosclerotic plaque Authors - Fu-Jun Liao View Article
mouse	WB,IHC	J Pathol Effects of long-term ethanol consumption and Aldh1b1 depletion on intestinal tumourigenesis in mice. Authors - Mike F Müller View Article
mouse	WB	J Ethnopharmacol Multi-omics and chemical profiling approaches to understand the material foundation and pharmacological mechanism of Sophorae Tonkinensis Radix et Rhizome-induced liver injury in mice Authors - Si-Wei Rao View Article
human	WB	Chin Med Shaoyao Decoction reduced T lymphocyte activation by regulating of intestinal flora and 5-hydroxytryptamine metabolism in ulcerative colitis Authors - Jianhua Zhen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALDH1B1 Polyclonal antibody

ALDH1B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HepG2 using 15560-1-AP

WB analysis of K-562 using 15560-1-AP

WB analysis of HepG2 using 15560-1-AP

WB analysis of mouse brain using 15560-1-AP

WB analysis of HepG2 using 15560-1-AP

WB analysis of K-562 using 15560-1-AP

IP experiment of mouse brain using 15560-1-AP

IHC staining of human testis using 15560-1-AP

IHC staining of human testis using 15560-1-AP

IF Staining of HepG2 using 15560-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.

FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.

Identification and experimental validation of KMO as a critical immune-associated mitochondrial gene in unstable atherosclerotic plaque

Effects of long-term ethanol consumption and Aldh1b1 depletion on intestinal tumourigenesis in mice.

Multi-omics and chemical profiling approaches to understand the material foundation and pharmacological mechanism of Sophorae Tonkinensis Radix et Rhizome-induced liver injury in mice

Shaoyao Decoction reduced T lymphocyte activation by regulating of intestinal flora and 5-hydroxytryptamine metabolism in ulcerative colitis