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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALDH1B1 Polyclonal antibody

ALDH1B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22220-1-AP

Synonyms

EC:1.2.1.3, ALDHX, ALDH5, Aldehyde dehydrogenase X, mitochondrial, Aldehyde dehydrogenase 5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ALDH1B1 Polyclonal antibody (22220-1-AP)

    WB analysis of HeLa using 22220-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ALDH1B1 Polyclonal antibody (22220-1-AP)

    WB analysis of mouse brain using 22220-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ALDH1B1 Polyclonal antibody (22220-1-AP)

    WB analysis of HepG2 using 22220-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ALDH1B1 Polyclonal antibody (22220-1-AP)

    WB analysis of mouse testis using 22220-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using ALDH1B1 Polyclonal antibody (22220-1-AP)

    WB analysis of rat brain using 22220-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ALDH1B1 Polyclonal antibody (22220-1-AP)

    IHC staining of human colon cancer using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ALDH1B1 Polyclonal antibody (22220-1-AP)

    IHC staining of human ovary tumor using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH1B1 Polyclonal antibody (22220-1-AP)

    IHC staining of human liver using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using ALDH1B1 Polyclonal antibody (22220-1-AP)

    IHC staining of rat kidney using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using ALDH1B1 Polyclonal antibody (22220-1-AP)

    IHC staining of rat kidney using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ALDH1B1 Polyclonal antibody (22220-1-AP)

    IHC staining of human colon cancer using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH1B1 Polyclonal antibody (22220-1-AP)

    IHC staining of human liver using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 22220-1-AP (ALDH1B1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH1B1 Polyclonal antibody (22220-1-AP)

    IHC staining of human liver using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 22220-1-AP (ALDH1B1 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ALDH1B1 Polyclonal antibody (22220-1-AP)

    IF Staining of MCF-7 using 22220-1-AP

    Immunofluorescent analysis of MCF-7 cells, using ALDH1B1 antibody 22220-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman ovary tumor tissue, human colon cancer tissue, human liver tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22220-1-AP targets ALDH1B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1B1 fusion protein Ag17557 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 1 family, member B1
Calculated molecular weight 517 aa, 57 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC001619
Gene symbol ALDH1B1
Gene ID (NCBI) 219
RRIDAB_2879035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112). This antibody is specific to ALDH1B1.

Protocols

Product Specific Protocols
WB protocol for ALDH1B1 antibody 22220-1-APDownload protocol
IHC protocol for ALDH1B1 antibody 22220-1-APDownload protocol
IF protocol for ALDH1B1 antibody 22220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Oncotarget

Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.

Authors - Hao-Long Zeng
View Article
humanIHC

Transl Cancer Res

ALDH1B1 predicts poor survival for locally advanced nasopharyngeal carcinoma patients.

Authors - Tao Zhu
View Article