Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue, mouse liver, rat liver |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 5 publications below |
| IF | See 5 publications below |
Product Information
17390-1-AP targets ALDH1L1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ALDH1L1 fusion protein Ag11285 相同性解析による交差性が予測される生物種 |
| Full Name | aldehyde dehydrogenase 1 family, member L1 |
| Calculated molecular weight | 99 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC027241 |
| Gene Symbol | ALDH1L1 |
| Gene ID (NCBI) | 10840 |
| RRID | AB_2878401 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75891 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALDH1L1 antibody 17390-1-AP | Download protocol |
| IHC protocol for ALDH1L1 antibody 17390-1-AP | Download protocol |
| IF protocol for ALDH1L1 antibody 17390-1-AP | Download protocol |
| IP protocol for ALDH1L1 antibody 17390-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Autologous exosome facilitates load and target delivery of bioactive peptides to repair spinal cord injury | ||
J Pineal Res Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis. | ||
Nat Commun Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain. | ||
NPJ Parkinsons Dis Reduced astrocytic reactivity in human brains and midbrain organoids with PRKN mutations. | ||