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Validation Data Gallery

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  • Western Blot (WB) analysis of human milk using ALDH1L1 Monoclonal antibody (68018-1-Ig)

    WB analysis of human milk using 68018-1-Ig

    human milk were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using ALDH1L1 Monoclonal antibody (68018-1-Ig)

    WB analysis of pig liver using 68018-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using ALDH1L1 Monoclonal antibody (68018-1-Ig)

    WB analysis of rat liver using 68018-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ALDH1L1 Monoclonal antibody (68018-1-Ig)

    WB analysis of mouse liver using 68018-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ALDH1L1 Monoclonal antibody (68018-1-Ig)

    IHC staining of mouse brain using 68018-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ALDH1L1 Monoclonal antibody (68018-1-Ig)

    IHC staining of mouse brain using 68018-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:32000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ALDH1L1 Monoclonal antibody (68018-1-Ig)

    IF Staining of mouse brain using 68018-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using ALDH1L1 antibody (68018-1-Ig, Clone: 1C1A3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3).

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ALDH1L1 Monoclonal antibody

ALDH1L1 Monoclonal Antibody for WB, IHC, IF-Fro, ELISA
Cat No. 68018-1-Ig
Clone No.1C1A3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-Fro, ELISA

Synonyms

10 FTHFDH, 10-FTHFDH, 1C1A3, ALDH1L 1, Cytosolic 10-formyltetrahydrofolate dehydrogenase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman colostrum, pig liver tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-Fro detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:16000-1:64000
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68018-1-Ig targets ALDH1L1 in WB, IHC, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALDH1L1 fusion protein Ag13190 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 1 family, member L1
Calculated molecular weight 99 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC027241
Gene Symbol ALDH1L1
Gene ID (NCBI) 10840
RRIDAB_2918764
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75891
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.

Protocols

Product Specific Protocols
WB protocol for ALDH1L1 antibody 68018-1-IgDownload protocol
IHC protocol for ALDH1L1 antibody 68018-1-IgDownload protocol
IF protocol for ALDH1L1 antibody 68018-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols