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KD/KO Validated

ALDH2 Polyclonal antibody

Name: ALDH2 Polyclonal antibody
Brand: Proteintech
SKU: 15310-1-AP
Rating: 5.00 (1 reviews)

ALDH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 15310-1-AP

Synonyms

ALDHI, ALDH-E2, ALDHE2, ALDH E2, ALDH class 2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 15310-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15310-1-AP (ALDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 15310-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15310-1-AP (ALDH2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15310-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15310-1-AP (ALDH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15310-1-AP
IP result of anti-ALDH2 (IP:15310-1-AP, 4ug; Detection:15310-1-AP 1:8000) with HepG2 cells lysate 2040 ug.
IHC staining of human liver using 15310-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15310-1-AP (ALDH2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 15310-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15310-1-AP (ALDH2 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon using 15310-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15310-1-AP (ALDH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 15310-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15310-1-AP (ALDH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 15310-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ALDH2 antibody (15310-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of A549 using 15310-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ALDH2 antibody (15310-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A549 using 15310-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 15310-1-AP (ALDH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 15310-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, HEK-293 cells, human heart tissue, mouse testis tissue, rat testis tissue, HepG2 cells, HuH-7 cells, mouse kidney tissue, mouse liver tisue, rat kidney tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 47 publications below
IHC	See 5 publications below
IF	See 6 publications below
IP	See 3 publications below
CoIP	See 2 publications below

Product Information

15310-1-AP targets ALDH2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ALDH2 fusion protein Ag7452 相同性解析による交差性が予測される生物種
Full Name	aldehyde dehydrogenase 2 family (mitochondrial)
Calculated molecular weight	56 kDa
Observed molecular weight	50-55 kDa
GenBank accession number	BC002967
Gene symbol	ALDH2
Gene ID (NCBI)	217
RRID	AB_2224185
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

Protocols

Product Specific Protocols
WB protocol for ALDH2 antibody 15310-1-AP	Download protocol
IHC protocol for ALDH2 antibody 15310-1-AP	Download protocol
IF protocol for ALDH2 antibody 15310-1-AP	Download protocol
IP protocol for ALDH2 antibody 15310-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Cancer Discov Transcriptional Silencing of ALDH2 Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia. Authors - Zhaolin Yang KO Validated View Article
human	WB	Blood Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency. Authors - Anfeng Mu KO Validated View Article
human,mouse	WB,IHC	Mol Cell Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans. Authors - Felix A Dingler KO Validated View Article
mouse	WB	Mol Cell Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome. Authors - Nina Oberbeck View Article
human,mouse	WB,IP,IF	J Clin Invest Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. Authors - Shanshan Zhong KO Validated View Article
human,mouse	WB,IF,IP	EMBO J p53 protects against alcoholic fatty liver disease via ALDH2 inhibition Authors - Pengbo Yao View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Hua (Verified Customer) (03-26-2021)

Excellent antibody.

Applications: Western Blot
Primary Antibody Dilution: 1:2000
Cell Tissue Type: human liver, mouse liver, mouse brain / HepG2

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALDH2 Polyclonal antibody

ALDH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 15310-1-AP

WB analysis using 15310-1-AP

WB analysis of human heart using 15310-1-AP

IP experiment of HepG2 using 15310-1-AP

IHC staining of human liver using 15310-1-AP

IHC staining of human liver using 15310-1-AP

IHC staining of human colon using 15310-1-AP

IHC staining of human colon using 15310-1-AP

IF Staining of A549 using 15310-1-AP

IF Staining of A549 using 15310-1-AP

IF Staining of A549 using 15310-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Transcriptional Silencing of ALDH2 Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia.

Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency.

Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans.

Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

p53 protects against alcoholic fatty liver disease via ALDH2 inhibition

Reviews