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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ALDH2 Monoclonal antibody (68237-1-Ig)

    WB analysis of mouse liver using 68237-1-Ig (same clone as 68237-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68237-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using ALDH2 Monoclonal antibody (68237-1-Ig)

    WB analysis of pig liver using 68237-1-Ig (same clone as 68237-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68237-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ALDH2 Monoclonal antibody (68237-1-Ig)

    WB analysis of HepG2 using 68237-1-Ig (same clone as 68237-1-PBS)

    WB result of ALDH2 antibody (68237-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH2 transfected HepG2 cells. This data was developed using the same antibody clone with 68237-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit liver tissue using ALDH2 Monoclonal antibody (68237-1-Ig)

    WB analysis of rabbit liver using 68237-1-Ig (same clone as 68237-1-PBS)

    rabbit liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68237-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using ALDH2 Monoclonal antibody (68237-1-Ig)

    WB analysis of rat liver using 68237-1-Ig (same clone as 68237-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68237-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ALDH2 Monoclonal antibody, PBS Only

ALDH2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68237-1-PBS
Clone No.2G10F4

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, Indirect ELISA

Synonyms

ALDH class 2, ALDH E2, ALDH2, ALDHI, ALDM

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68237-1-PBS targets ALDH2 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ALDH2 fusion protein Ag7463 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 2 family (mitochondrial)
Calculated molecular weight 56 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC002967
Gene Symbol ALDH2
Gene ID (NCBI) 217
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05091
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.