Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 15090-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15090-1-AP (ALDH3A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 15090-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15090-1-AP (ALDH3A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15090-1-AP
IP result of anti-ALDH3A2 (IP:15090-1-AP, 4ug; Detection:15090-1-AP 1:1000) with HEK-293 cells lysate 3000ug.
IP experiment of HEK-293 using 15090-1-AP
IP result of anti-ALDH3A2 (IP:15090-1-AP, 4ug; Detection:15090-1-AP 1:6000) with HEK-293 cells lysate 680 ug.
IHC staining of human liver cancer using 15090-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15090-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15090-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15090-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15090-1-AP
Immunofluorescent analysis of Hela cells, using ALDH3A2 antibody 15090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 15090-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDH3A2 antibody (15090-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

ALDH3A2 Polyclonal antibody

ALDH3A2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15090-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

SLS, FALDH, EC:1.2.1.3, ALDH10, Aldehyde dehydrogenase 10

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293T cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 5 publications below
IHC	See 2 publications below

Product Information

15090-1-AP targets ALDH3A2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ALDH3A2 fusion protein Ag1687 相同性解析による交差性が予測される生物種
Full Name	aldehyde dehydrogenase 3 family, member A2
Calculated molecular weight	55 kDa
Observed molecular weight	50-57 kDa
GenBank accession number	BC002430
Gene Symbol	ALDH3A2
Gene ID (NCBI)	224
RRID	AB_2224316
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P51648
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ALDH3A2 antibody 15090-1-AP	Download protocol
IHC protocol for ALDH3A2 antibody 15090-1-AP	Download protocol
IF protocol for ALDH3A2 antibody 15090-1-AP	Download protocol
IP protocol for ALDH3A2 antibody 15090-1-AP	Download protocol
FC protocol for ALDH3A2 antibody 15090-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Transl Med Deficiency of SDHC promotes metastasis by reprogramming fatty acid metabolism in colorectal cancer Authors - Zhuoyu Ding View Article
rat	IHC	J Cell Mol Med Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis. Authors - Wei Chen View Article
	IHC	Cells Aldehyde Dehydrogenase, a Therapeutic Target in Chordoma: Analysis in 3D Cellular Models. Authors - Marie-Anaïs Locquet View Article
mouse	WB	J Biol Chem Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways. Authors - Pamela A Young View Article
human	WB	Int J Mol Sci Targeting Retinaldehyde Dehydrogenases to Enhance Temozolomide Therapy in Glioblastoma Authors - Rafael Jiménez View Article
		Front Med (Lausanne) Synthetic antibody-derived immunopeptide provides neuroprotection in glaucoma through molecular interaction with retinal protein histone H3.1 Authors - Kristian Nzogang Fomo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15090-1-AP

WB analysis using 15090-1-AP

IP experiment of HEK-293 using 15090-1-AP

IP experiment of HEK-293 using 15090-1-AP

IHC staining of human liver cancer using 15090-1-AP

IHC staining of human liver cancer using 15090-1-AP

IHC staining of human liver cancer using 15090-1-AP

IHC staining of human liver cancer using 15090-1-AP

IF Staining of HeLa using 15090-1-AP

IF Staining of HeLa using 15090-1-AP

ALDH3A2 Polyclonal antibody

ALDH3A2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Deficiency of SDHC promotes metastasis by reprogramming fatty acid metabolism in colorectal cancer

Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis.

Aldehyde Dehydrogenase, a Therapeutic Target in Chordoma: Analysis in 3D Cellular Models.

Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.

Targeting Retinaldehyde Dehydrogenases to Enhance Temozolomide Therapy in Glioblastoma

Synthetic antibody-derived immunopeptide provides neuroprotection in glaucoma through molecular interaction with retinal protein histone H3.1