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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALDH8A1 Polyclonal antibody

ALDH8A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21602-1-AP

Synonyms

EC:1.2.1.32, ALDH8A 1, ALDH12, 2-aminomuconic semialdehyde dehydrogenase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDH8A1 Polyclonal antibody (21602-1-AP)

    WB analysis using 21602-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using ALDH8A1 Polyclonal antibody (21602-1-AP)

    WB analysis of L02 using 21602-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using ALDH8A1 Polyclonal antibody (21602-1-AP)

    WB analysis of L02 using 21602-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ALDH8A1 Polyclonal antibody (21602-1-AP)

    WB analysis of mouse kidney using 21602-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ALDH8A1 Polyclonal antibody (21602-1-AP)

    IHC staining of human hepatocirrhosis using 21602-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ALDH8A1 Polyclonal antibody (21602-1-AP)

    IHC staining of human hepatocirrhosis using 21602-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ALDH8A1 Polyclonal antibody (21602-1-AP)

    IF Staining of HepG2 using 21602-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inL02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21602-1-AP targets ALDH8A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH8A1 fusion protein Ag16214 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 8 family, member A1
Calculated molecular weight 487 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC113862
Gene symbol ALDH8A1
Gene ID (NCBI) 64577
RRIDAB_10733887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ALDH8A1 antibody 21602-1-APDownload protocol
IHC protocol for ALDH8A1 antibody 21602-1-APDownload protocol
IF protocol for ALDH8A1 antibody 21602-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols