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  • Featured Product
  • KD/KO Validated

ALDOA Polyclonal antibody

ALDOA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 11217-1-AP

Synonyms

Muscle-type aldolase, Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDOA Polyclonal antibody (11217-1-AP)

    WB analysis using 11217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ALDOA Polyclonal antibody (11217-1-AP)

    WB analysis of HeLa using 11217-1-AP

    WB result of ALDOA antibody (11217-1-AP; 1:240000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDOA transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using ALDOA Polyclonal antibody (11217-1-AP)

    WB analysis using 11217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ALDOA Polyclonal antibody (11217-1-AP)

    WB analysis using 11217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ALDOA Polyclonal antibody (11217-1-AP)

    WB analysis using 11217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ALDOA Polyclonal antibody (11217-1-AP)

    IP experiment of HepG2 using 11217-1-AP

    IP result of anti-ALDOA (IP:11217-1-AP, 3ug; Detection:11217-1-AP 1:1000) with HepG2 cells lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ALDOA Polyclonal antibody (11217-1-AP)

    IHC staining of human colon cancer using 11217-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11217-1-AP (ALDOA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ALDOA Polyclonal antibody (11217-1-AP)

    IHC staining of human colon cancer using 11217-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11217-1-AP (ALDOA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ALDOA Polyclonal antibody (11217-1-AP)

    IHC staining of human colon cancer using 11217-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ALDOA Polyclonal antibody (11217-1-AP)

    IHC staining of human colon cancer using 11217-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ALDOA Polyclonal antibody (11217-1-AP)

    IHC staining of human skeletal muscle using 11217-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ALDOA Polyclonal antibody (11217-1-AP)

    IHC staining of human skeletal muscle using 11217-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ALDOA Polyclonal antibody (11217-1-AP)

    IHC staining of human lung cancer using 11217-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ALDOA Polyclonal antibody (11217-1-AP)

    IHC staining of human lung cancer using 11217-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ALDOA Polyclonal antibody (11217-1-AP)

    IF Staining of HeLa using 11217-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDOA antibody (11217-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ALDOA Polyclonal antibody (11217-1-AP)

    IF Staining of HeLa using 11217-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody (11217-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, A549 cells, Jurkat cells, mouse liver tissue, rat liver tissue, HepG2 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11217-1-AP targets ALDOA in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDOA fusion protein Ag1726 相同性解析による交差性が予測される生物種
Full Name aldolase A, fructose-bisphosphate
Calculated molecular weight 39 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC016800
Gene symbol ALDOA
Gene ID (NCBI) 226
RRIDAB_2224626
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

Protocols

Product Specific Protocols
WB protocol for ALDOA antibody 11217-1-APDownload protocol
IHC protocol for ALDOA antibody 11217-1-APDownload protocol
IF protocol for ALDOA antibody 11217-1-APDownload protocol
IP protocol for ALDOA antibody 11217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li
View Article
mouseWB

Nat Neurosci

Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.

Authors - Xiaoxiang Dong
View Article
mouseWB

Dev Cell

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.

Authors - Vinay Bulusu
View Article
human,mouseWB

Int J Biol Sci

Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway

Authors - Yunfeng Guan
View Article
humanWB

Cell Death Dis

The ubiquitin-like protein UBTD1 promotes colorectal cancer progression by stabilizing c-Myc to upregulate glycolysis

Authors - Liqin Zhao
View Article
humanIF

Mol Ther

Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.

Authors - Junyi Hu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

K (Verified Customer) (12-20-2020)

Worked well for IHC at 1:200 dilution

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: mouse liver tissues and rat liver