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ALDOA Recombinant antibody

ALDOA Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Pig, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F4

Cat no : 80837-1-RR

Synonyms

Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A, ALDA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDOA Recombinant antibody (80837-1-RR)

    WB analysis using 80837-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80837-1-RR (ALDOA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ALDOA Recombinant antibody (80837-1-RR)

    WB analysis of HEK-293 using 80837-1-RR

    WB result of ALDOA antibody (80837-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDOA transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ALDOA Recombinant antibody (80837-1-RR)

    IF Staining of HeLa using 80837-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDOA antibody (80837-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ALDOA Recombinant antibody (80837-1-RR)

    IF Staining of HeLa using 80837-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody (80837-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 80837-1-RR

    Affinity and Kinetic Characterization of 80837-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80837-1-RR against Human ALDOA were performed. The affinity constant is 1.34 nM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells, HSC-T6 cells, pig liver tissue, pig skeletal muscle tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80837-1-RR targets ALDOA in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig, rat samples.

Tested Reactivity Human, Mouse, Pig, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALDOA fusion protein Ag1726 相同性解析による交差性が予測される生物種
Full Name aldolase A, fructose-bisphosphate
Calculated molecular weight 39 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC016800
Gene symbol ALDOA
Gene ID (NCBI) 226
RRIDAB_2918914
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

Protocols

Product Specific Protocols
WB protocol for ALDOA antibody 80837-1-RRDownload protocol
IF protocol for ALDOA antibody 80837-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols