• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALDOB Monoclonal antibody

ALDOB Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D2E4

Cat no : 68396-1-Ig

Synonyms

ALDB, ALDOB, Liver type aldolase

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ALDOB Monoclonal antibody (68396-1-Ig)

    WB analysis using 68396-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ALDOB Monoclonal antibody (68396-1-Ig)

    WB analysis using 68396-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ALDOB Monoclonal antibody (68396-1-Ig)

    WB analysis using 68396-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human urine sample using ALDOB Monoclonal antibody (68396-1-Ig)

    WB analysis of human urine using 68396-1-Ig

    human urine sample were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ALDOB Monoclonal antibody (68396-1-Ig)

    IF Staining of HepG2 using 68396-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALDOB antibody (68396-1-Ig, Clone: 3D2E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inrat kidney tissue, human urine sample, rabbit liver tissue, rat liver tissue, mouse liver tissue, mouse kidney tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68396-1-Ig targets ALDOB in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ALDOB fusion protein Ag12823 相同性解析による交差性が予測される生物種
Full Name aldolase B, fructose-bisphosphate
Calculated molecular weight 316 aa, 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC029399
Gene symbol ALDOB
Gene ID (NCBI) 229
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.

Protocols

Product Specific Protocols
WB protocol for ALDOB antibody 68396-1-IgDownload protocol
IF protocol for ALDOB antibody 68396-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols