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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALG11 Polyclonal antibody

ALG11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27085-1-AP

Synonyms

KIAA0266, GT8, Glycolipid 2-alpha-mannosyltransferase, GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase, EC:2.4.1.131

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ALG11 Polyclonal antibody (27085-1-AP)

    WB analysis of mouse brain using 27085-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27085-1-AP (ALG11 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using ALG11 Polyclonal antibody (27085-1-AP)

    WB analysis of rat brain using 27085-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 27085-1-AP (ALG11 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using ALG11 Polyclonal antibody (27085-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27085-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27085-1-AP (ALG11 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27085-1-AP targets ALG11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALG11 fusion protein Ag21123 相同性解析による交差性が予測される生物種
Full Name asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast)
Calculated molecular weight 328 aa, 37 kDa
Observed molecular weight 50 kDa~56 kDa
GenBank accession numberBC010857
Gene symbol ALG11
Gene ID (NCBI) 440138
RRIDAB_2880750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ALG11 antibody 27085-1-APDownload protocol
IHC protocol for ALG11 antibody 27085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols