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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALG13 Polyclonal antibody

ALG13 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28738-1-AP

Synonyms

ALG13, CXorf45, FLJ23018, GLT28D1, MDS031, YGL047W

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALG13 Polyclonal antibody (28738-1-AP)

    WB analysis using 28738-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 28738-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ALG13 Polyclonal antibody (28738-1-AP)

    IHC staining of mouse brain using 28738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28738-1-AP (ALG13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ALG13 Polyclonal antibody (28738-1-AP)

    IHC staining of mouse brain using 28738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28738-1-AP (ALG13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ALG13 Polyclonal antibody (28738-1-AP)

    IF Staining of mouse brain using 28738-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ALG13 antibody (28738-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28738-1-AP targets ALG13 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALG13 fusion protein Ag30299 相同性解析による交差性が予測される生物種
Full Name asparagine-linked glycosylation 13 homolog
Calculated molecular weight 165 aa, 18 kDa
Observed molecular weight130-135,18 kDa
GenBank accession numberBC005336
Gene symbol ALG13
Gene ID (NCBI) 79868
RRIDAB_2918198
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ALG13 antibody 28738-1-APDownload protocol
IHC protocol for ALG13 antibody 28738-1-APDownload protocol
IF protocol for ALG13 antibody 28738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

An in vitro assay for enzymatic studies on human ALG13/14 heterodimeric UDP-N-acetylglucosamine transferase

Authors - Chun-Di Wang
View Article