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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALG9 Polyclonal antibody

ALG9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29218-1-AP

Synonyms

EC:2.4.1.259, Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase, Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase, DIBD1, Asparagine-linked glycosylation protein 9 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALG9 Polyclonal antibody (29218-1-AP)

    WB analysis using 29218-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29218-1-AP (ALG9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ALG9 Polyclonal antibody (29218-1-AP)

    IHC staining of human stomach cancer using 29218-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29218-1-AP (ALG9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ALG9 Polyclonal antibody (29218-1-AP)

    IHC staining of human stomach cancer using 29218-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29218-1-AP (ALG9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells, THP-1 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29218-1-AP targets ALG9 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALG9 fusion protein Ag30489 相同性解析による交差性が予測される生物種
Full Name asparagine-linked glycosylation 9, alpha-1,2-mannosyltransferase homolog (S. cerevisiae)
Calculated molecular weight 70 kDa
Observed molecular weight70 kDa
GenBank accession numberBC009255
Gene symbol ALG9
Gene ID (NCBI) 79796
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALG9 encodes an endoplasmic reticulum enzyme that builds N-glycans, the third ER protein-encoding polycystic disease gene after GANAB and DNAJB11. Autosomal recessive loss of ALG9 results in a severe congenital disorder of glycosylation (CDG) with a multiorgan phenotype that includes kidney cysts (PMID: 31395617). Western blot analysis detected ALG9 at an apparent molecular mass of 70 kDa.

Protocols

Product Specific Protocols
WB protocol for ALG9 antibody 29218-1-APDownload protocol
IHC protocol for ALG9 antibody 29218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols