Validation Data Gallery
Tested Applications
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
60321-1-Ig targets ALK in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ALK fusion protein Ag21493 相同性解析による交差性が予測される生物種 |
| Full Name | anaplastic lymphoma receptor tyrosine kinase |
| Calculated molecular weight | 1620 aa, 176 kDa |
| GenBank accession number | NM_004304 |
| Gene Symbol | ALK |
| Gene ID (NCBI) | 238 |
| RRID | AB_2881432 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UM73 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The EML4 (echinoderm microtubule-associated protein-like 4)-ALK fusion protein have been described in non-small-cell lung cancer (NSCLC), this transforming fusion kinase is a promising candidate for a therapeutic target as well as for a diagnostic molecular marker in NSCLC (PMID: 17625570).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ALK antibody 60321-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Eur J Med Chem Discovery of 2,4-pyrimidinediamine derivatives as potent dual inhibitors of ALK and HDAC. | ||
iScience DAB2IP suppresses invadopodia formation through destabilizing ALK by interacting with USP10 in breast cancer |