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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALKBH2 Polyclonal antibody (17809-1-AP)

    WB analysis using 17809-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17809-1-AP (ALKBH2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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ALKBH2 Polyclonal antibody

ALKBH2 Polyclonal Antibody for WB, ELISA
Cat No. 17809-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ABH2, ALKBH2, Oxy DC1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17809-1-AP targets ALKBH2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALKBH2 fusion protein Ag12108 相同性解析による交差性が予測される生物種
Full Name alkB, alkylation repair homolog 2 (E. coli)
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight30-35 kDa
GenBank accession numberBC070489
Gene Symbol ALKBH2
Gene ID (NCBI) 121642
RRIDAB_3085541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6NS38
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Escherichia coli AlkB is a DNA repair enzyme that represents the founding member of the AlkB family of non-heme iron-dependent dioxygenases. The human genome encodes nine AlkB homologs termed AlkB homolog 1 through 8 (ALKBH1 through 8) plus a ninth member termed fat mass and obesity associated (FTO). Bacterial AlkB as well as human ALKBH2 and ALKBH3 catalyze the direct reversal of DNA methylation damage through a distinct oxidative demethylation reaction. Importantly, ALKBH2 has been shown to play a role in DNA alkylation damage repair in human, mouse and plant cells. Full-length ALKBH2 has a molecular weight of about 30kDa and ALKBH2 variant encoded by an alternatively spliced transcript with a frameshift deletion of the C-terminal dioxygenase domain has a molecular weight of about 25kDa (PMID:22079122).

Protocols

Product Specific Protocols
WB protocol for ALKBH2 antibody 17809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols