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  • Featured Product
  • KD/KO Validated

ALKBH3 Polyclonal antibody

ALKBH3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12292-1-AP

Synonyms

ABH3, ALKBH3, DEPC 1, DEPC1, PCA1, Prostate cancer antigen 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALKBH3 Polyclonal antibody (12292-1-AP)

    WB analysis using 12292-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    WB analysis of mouse kidney using 12292-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    WB analysis of mouse heart using 12292-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ALKBH3 Polyclonal antibody (12292-1-AP)

    WB analysis using 12292-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of human lung cancer using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of human prostate cancer using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of human lung cancer using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of human pancreas cancer using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of rat stomach using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of mouse stomach using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of human testis using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of human testis using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inJurkat cells, mouse kidney tissue, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman lung cancer tissue, human testis tissue, human prostate cancer tissue, human pancreas cancer tissue, rat stomach tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12292-1-AP targets ALKBH3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALKBH3 fusion protein Ag2982 相同性解析による交差性が予測される生物種
Full Name alkB, alkylation repair homolog 3 (E. coli)
Calculated molecular weight 286 aa, 33 kDa
Observed molecular weight 22-25 kDa, 33kDa
GenBank accession numberBC015155
Gene symbol ALKBH3
Gene ID (NCBI) 221120
RRIDAB_11125161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.

Protocols

Product Specific Protocols
WB protocol for ALKBH3 antibody 12292-1-APDownload protocol
IHC protocol for ALKBH3 antibody 12292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Gastroenterol Hepatol

ALKBH3 expression is associated with tumor recurrence and unfavorable prognosis in hepatocellular carcinoma.

Authors - Qian Wang
  • KD Validated
View Article
humanWB

Cell Biosci

m1A demethylase Alkbh3 regulates neurogenesis through m1A demethylation of Mmp15 mRNA

Authors - Huan Wang
View Article
humanIHC

Biomedicines

The Roles of AGTRAP, ALKBH3, DIVERSIN, NEDD8 and RRM1 in Glioblastoma Pathophysiology and Prognosis

Authors - Claudia Alexandra Dumitru
View Article
humanIHC

Clin Transl Med

PUS7-dependent pseudouridylation of ALKBH3 mRNA inhibits gastric cancer progression

Authors - Yongxia Chang
View Article