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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALKBH3 Recombinant antibody

ALKBH3 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241650C3

Cat no : 84314-3-RR

Synonyms

DEPC-1, DEPC1, DEPC 1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, ABH3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALKBH3 Recombinant antibody (84314-3-RR)

    WB analysis using 84314-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84314-3-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ALKBH3 Recombinant antibody (84314-3-RR)

    WB analysis using 84314-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84314-3-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ALKBH3 Recombinant antibody (84314-3-RR)

    IF Staining of A431 using 84314-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ALKBH3 antibody (84314-3-RR, Clone: 241650C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using ALKBH3 Recombinant antibody (84314-3-RR)

    FC experiment of HeLa using 84314-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-3-RR, Clone:241650C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of PC-3 cells using ALKBH3 Recombinant antibody (84314-3-RR)

    FC experiment of PC-3 using 84314-3-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-3-RR, Clone:241650C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84314-3-RR

    Affinity and Kinetic Characterization of 84314-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84314-3-RR against Human ALKBH3 were performed. The affinity constant is 1.42 nM.

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Tested Applications

Positive WB detected inJurkat cells, mouse kidney tissue, mouse heart tissue, rat heart tissue
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inPC-3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84314-3-RR is ALKBH3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALKBH3 fusion protein Ag2982 相同性解析による交差性が予測される生物種
Full Name alkB, alkylation repair homolog 3 (E. coli)
Calculated molecular weight 286 aa, 33 kDa
Observed molecular weight19-25 kDa
GenBank accession numberBC015155
Gene symbol ALKBH3
Gene ID (NCBI) 221120
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.

Protocols

Product Specific Protocols
WB protocol for ALKBH3 antibody 84314-3-RRDownload protocol
IF protocol for ALKBH3 antibody 84314-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols