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KD/KO Validated

ALKBH4 Polyclonal antibody

Name: ALKBH4 Polyclonal antibody
Brand: Proteintech
SKU: 19882-1-AP

ALKBH4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 19882-1-AP

Synonyms

ABH4, ALKBH4

Validation Data Gallery

Filter:

AllWBIF/ICC

WB analysis of mouse kidney using 19882-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 19882-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 19882-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 19882-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 19882-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALKBH4 antibody (19882-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 19882-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse kidney tissue, MCF-7 cells, K-562 cells, mouse ovary tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 5 publications below
IF	See 1 publications below

Product Information

19882-1-AP targets ALKBH4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ALKBH4 fusion protein Ag8041 相同性解析による交差性が予測される生物種
Full Name	alkB, alkylation repair homolog 4 (E. coli)
Calculated molecular weight	34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC002820
Gene symbol	ALKBH4
Gene ID (NCBI)	54784
RRID	AB_10792931
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ALKBH4 antibody 19882-1-AP	Download protocol
IF protocol for ALKBH4 antibody 19882-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Discov Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer. Authors - Chengheng Liao KD Validated View Article
human	WB,IF	Sci Rep ALKBH overexpression in head and neck cancer: potential target for novel anticancer therapy. Authors - Tomaš Pilžys KD Validated View Article
human	WB	Acta Biochim Pol Selected ALKBH dioxygenases are overexpressed in salivary gland tumours Authors - Michał Marcinkowski View Article
human,mouse	WB	Water (Basel) ALKBH4 Stabilization Is Required for Arsenic-Induced 6mA DNA Methylation Inhibition, Keratinocyte Malignant Transformation, and Tumorigenicity Authors - Yan-Hong Cui KD Validated View Article
pig	WB	Sci Rep The proteomic profile is altered but not repaired after bariatric surgery in type 2 diabetes pigs Authors - Karolina Ferenc View Article

製品

アプリケーション

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サポート

カスタム対応

プロモーション

企業情報

抗体

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サポートツール・試薬

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALKBH4 Polyclonal antibody

ALKBH4 Polyclonal Antibody for WB, IF/ICC, ELISA

Validation Data Gallery

WB analysis of mouse kidney using 19882-1-AP

WB analysis of MCF-7 using 19882-1-AP

WB analysis of K-562 using 19882-1-AP

WB analysis of mouse ovary using 19882-1-AP

IF Staining of HepG2 using 19882-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer.

ALKBH overexpression in head and neck cancer: potential target for novel anticancer therapy.

Selected ALKBH dioxygenases are overexpressed in salivary gland tumours

ALKBH4 Stabilization Is Required for Arsenic-Induced 6mA DNA Methylation Inhibition, Keratinocyte Malignant Transformation, and Tumorigenicity

The proteomic profile is altered but not repaired after bariatric surgery in type 2 diabetes pigs