Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human testis tissue, human lung cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 17 publications below |
WB | See 148 publications below |
IHC | See 20 publications below |
IF | See 13 publications below |
CoIP | See 1 publications below |
RIP | See 12 publications below |
Product Information
16837-1-AP targets ALKBH5 in WB, IHC, IF, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, chicken, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ALKBH5 fusion protein Ag10516 相同性解析による交差性が予測される生物種 |
Full Name | alkB, alkylation repair homolog 5 (E. coli) |
Calculated molecular weight | 458 aa, 51 kDa |
Observed molecular weight | 40-50 kDa |
GenBank accession number | BC062339 |
Gene Symbol | ALKBH5 |
Gene ID (NCBI) | 54890 |
RRID | AB_2242665 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q6P6C2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for ALKBH5 antibody 16837-1-AP | Download protocol |
IHC protocol for ALKBH5 antibody 16837-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Genet N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2. | ||
Nat Cell Biol The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA. | ||
Nat Commun Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma | ||
Nat Commun ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice |