• Featured Product
  • KD/KO Validated

ALKBH5 Polyclonal antibody

ALKBH5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 16837-1-AP

Synonyms

ABH5, ALKBH5, OFOXD, OFOXD1


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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16837-1-AP targets ALKBH5 in WB, IHC, IF, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALKBH5 fusion protein Ag10516 相同性解析による交差性が予測される生物種
Full Name alkB, alkylation repair homolog 5 (E. coli)
Calculated molecular weight 458 aa, 51 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC062339
Gene symbol ALKBH5
Gene ID (NCBI) 54890
RRIDAB_2242665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

Protocols

Product Specific Protocols
WB protocol for ALKBH5 antibody 16837-1-APDownload protocol
IHC protocol for ALKBH5 antibody 16837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Genet

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Authors - Yuan Li
humanIF

Cell Res

The m6A methylome of SARS-CoV-2 in host cells.

Authors - Jun'e Liu
  • KD Validated
mouseWB,RIP

Nat Cell Biol

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.

Authors - Xiang-Qian Gao
humanWB

Nat Commun

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis

Authors - Jian Zhong
human,mouseWB

Nat Commun

Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma

Authors - Weiwei Dai
mouseWB

Nat Commun

ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice

Authors - Tao Zhuang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

PK (Verified Customer) (07-08-2022)

GOOD

  • Applications: Western Blot
  • Cell Tissue Type: H9C2
ALKBH5 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:16837-1-AP)
FH

SU (Verified Customer) (03-22-2022)

  • Applications: Western Blot
  • Cell Tissue Type: H9C2
ALKBH5 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:16837-1-AP)
FH

ANIRBAN (Verified Customer) (04-07-2021)

Tried to validate the antibody (AlkBH5) in two different cell lines (786-o, HK2) using three siRNA constructs from IDT. Using the Proteintech antibody, I didn't see any evedence of KD in the lysates (see the figure attached). When I used the Cell Signaling antibody for AlkBH5 (#80283) I observed a clear evedence of KD in the same lysates that I mentioned before. scr=scrambled; si-1,2,3=siRNAs targeting AlkBH5.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2K
  • Cell Tissue Type: RCC cell lines
ALKBH5 Antibody Western Blot validation (1:2K dilution) in RCC cell lines (Cat no:16837-1-AP)
FH

Bill (Verified Customer) (12-23-2019)

This antibody has some unspecific band, but is ok to detect ALKBH5, siRNA validated.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: colerectal cancer cells
FH

Daniel (Verified Customer) (12-04-2019)

Staining of kidney proximal tubules

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse kidney (TRACK model)
ALKBH5 Antibody Immunohistochemistry, validation (1:200 dilution) in Mouse kidney (TRACK model) (Cat no:16837-1-AP)