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Validation Data Gallery

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  • Western Blot (WB) analysis of HSC-T6 cells using ALKBH5 Monoclonal antibody (67811-1-Ig)

    WB analysis of HSC-T6 using 67811-1-Ig (same clone as 67811-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using ALKBH5 Monoclonal antibody (67811-1-Ig)

    WB analysis of NIH/3T3 using 67811-1-Ig (same clone as 67811-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using ALKBH5 Monoclonal antibody (67811-1-Ig)

    WB analysis of 4T1 using 67811-1-Ig (same clone as 67811-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ALKBH5 Monoclonal antibody (67811-1-Ig)

    WB analysis of HeLa using 67811-1-Ig (same clone as 67811-1-PBS)

    WB result of ALKBH5 antibody (67811-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ALKBH5 Monoclonal antibody (67811-1-Ig)

    WB analysis of HepG2 using 67811-1-Ig (same clone as 67811-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ALKBH5 Monoclonal antibody (67811-1-Ig)

    WB analysis of Jurkat using 67811-1-Ig (same clone as 67811-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using ALKBH5 Monoclonal antibody (67811-1-Ig)

    WB analysis of K-562 using 67811-1-Ig (same clone as 67811-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ALKBH5 Monoclonal antibody (67811-1-Ig)

    IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ALKBH5 Monoclonal antibody (67811-1-Ig)

    IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ALKBH5 Monoclonal antibody, PBS Only

ALKBH5 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67811-1-PBS
Clone No.2D2B2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67811-1-PBS targets ALKBH5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ALKBH5 fusion protein Ag10490 相同性解析による交差性が予測される生物種
Full Name alkB, alkylation repair homolog 5 (E. coli)
Calculated molecular weight 458 aa, 51 kDa
Observed molecular weight40-44 kDa
GenBank accession numberBC062339
Gene Symbol ALKBH5
Gene ID (NCBI) 54890
RRIDAB_2918574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P6C2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.